PerkinElmer

Alpha试剂

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha试剂" 1-50954 产品与服务

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TRAIL (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human TNF-related apoptosis-inducing ligand (TRAIL) enables the quantitative determination of human TRAIL in buffered solution and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

Influenza A Nucleoprotein (NP) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for influenza A nucleoprotein (NP) enables the quantitative determination of nucleoprotein in buffered solution and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA SureFire Ultra kits

Phospho-eIF2α (Ser51) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ p-eIF2α (Ser51) assay is a sandwich immunoassay for quantitative detection of phospho-eIF2α (phosphorylated on Ser51) in cellular lysates using Alpha Technology.
AlphaPlex Samarium Acceptor Beads

Unconjugated AlphaPlex™ 545 Acceptor Beads

Unconjugated AlphaPlex 645 (Samarium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
AlphaLisa Analyte 2

Cry1Ab/1Ac, lyophilized (AlphaLISA)

Cry1Ab and Cry1Ac standards, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Cry1Ab/1Ac Detection Kit, but can be ordered separately.
AlphaLISA immunoassay kits

B7-H5/VISTA (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human V-domain Ig suppressor of T cell activation (VISTA) enables the quantitative determination of human VISTA (B7-H5, PD-1H) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Fc-fragment-conjugated Alpha Donor beads

Fc-fragment (human) conjugated Alpha Donor Beads

Alpha Donor beads conjugated to human IgG Fc fragment. These beads can be used to capture Fc binding proteins and receptors.

AlphaLISA Anti-unmodified Histone H3 Lysine 4 (H3K4) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 unmodified against lysine 4 (H3K4). These beads can be used for no-wash AlphaLISA epigenetic eraser assays.
AlphaScreen Acceptor Beads

AlphaScreen Unconjugated Acceptor Beads

Unconjugated AlphaScreen® Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaScreen Acceptor beads for use in Alpha no-wash assays.

IP-10 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
AlphaLisa Analyte 2

Transferrin (human), lyophilized (AlphaLISA)

Human Transferrin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays.This standard is already provided in the AlphaLISA Human transferrin kit, but can be ordered separately.
Assay principle of Alpha Terbium SureFire Ultra assay

Total Cofilin Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two targets in each well of an assay plate. This kit can be used in combination with the AlphaLISA SureFire Ultra phospho-cofilin assay to normalize to levels of total cofilin.
AlphaLisa Analyte 2

Leptin (mouse), lyophilized (AlphaLISA)

Mouse leptin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse leptin kit, but can be ordered separately.

Anti-HA AlphaLISA Acceptor Beads

AlphaLISA Acceptor beads conjugated to anti-HA (hemagglutinin) antibody. These beads can be used to capture HA-tagged proteins and peptides.

Anti-rat IgG (Fc specific) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-rat IgG antibody. These beads can be used to capture rat IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.
Assay principle for LANCE TR-FRET binding assay

CTLA4-CD80 AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between Human CTLA-4 and CD80, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding to CTLA-4/CD80.
AlphaLisa Analyte 2

N-cadherin (human), lyophilized (AlphaLISA)

Human N-cadherin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human N-cadherin Detection Kit, but can be ordered separately.

CCL5/RANTES (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-Cell Expressed, and Secreted (CCL5/RANTES) Detection Kit is designed for detection and quantitation of mouse CCL5/RANTES in serum, bronchial lavage fluid (BALF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT6 (Tyr641) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT6 (Tyr641) in a multiplexed assay.
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IL1β Detection Kit

The AlphaPlex™ 645 (Samarium) Human IL1β Detection Kit is designed for the quantitative determination of human insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

Transthyretin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human transthyretin enables the quantitative determination of human transthyretin (TTR) in buffer, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.
AlphaLISA immunoassay kits

Insulin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human insulin enables the detection and quantitation of insulin in serum, buffered solution or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). The antibodies in the kit are mouse monoclonal antibodies (clone numbers 8E2 and 3A6).
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total SLP-76 Assay Kit

The AlphaLISA® SureFire® Ultra Total SLP-76 assay is a sandwich immunoassay for quantitative detection of SLP-76 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
AlphaLISA immunoassay kits

CD40 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD40 enables the quantitative determination of human cluster of differentiation 40 protein in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT3 (Tyr705) assay is a sandwich style immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.
AlphaLisa Analyte 2

AST (human), lyophilized (AlphaLISA)

Human AST standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human AST Detection Kit, but can also be ordered separately.
AlphaLisa Analyte 2

Vimentin (human), liquid (AlphaLISA)

Human vimentin standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human Vimentin Detection Kit, but can also be ordered separately.
AlphaPlex-545-unconjugated

Unconjugated AlphaPlex-545 Acceptor Beads

Unconjugated AlphaPlex 545 (Terbium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
AlphaLISA immunoassay kits

IL-25 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-25 enables the quantitative determination of human interleukin 25 (IL-25) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

Anti-IgG1 AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-human IgG1. These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for antibody binding studies, pharmacokinetics (PK), or detection of human IgG1 in different sample matrices, including monkey serum.
AlphaLISA immunoassay kits

Chloramphenicol Biotin-free AlphaLISA Detection Kit

The AlphaLISA® biotin-free immunoassay kit for chloramphenicol enables the quantitative determination of chloramphenicol in buffered solution, milk and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The kit utilizes a Digoxigenin (DIG) / Anti-DIG interaction as opposed to the traditional Streptavidin/Biotin interaction. This enables optimal performance when working with biotin-rich media (e.g. RPMI) or samples containing endogenous biotin.
AlphaLISA immunoassay kits

TNFα (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human TNFα Biotin-Free Detection Kit is designed for the quantitative detection of human TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
AlphaLISA immunoassay kits

FcRn (human) AlphaLISA Binding Kit

The AlphaLISA human FcRn binding kit is designed for the detection of binding between FcRn and human IgG using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics using a competition assay format.

CD276 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD276 enables the quantitative determination of human Cluster of Differentiation 276 (CD276, B7-H3) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLisa Analyte 2

D-dimer (human), lyophilized (AlphaLISA)

Human D-dimer standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human D-dimer kit, but can be ordered separately.

FcγR2A/CD32a (167R) AlphaLISA Binding Kit

This kit is designed for the detection of binding between FCGR2A (CD32a) (167R) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics by using competitive binding.
AlphaLISA immunoassay kits

PIP Collagen (human) AlphaLISA Detection Kit

The AlphaLISA® Human PIP Detection Kit is designed for detection and quantitation of Human Procollagen Type I C-peptide (PIP) in human serum, plasma, cell culture supernatants, cell lysate, and tissue homogenates using a homogeneous (no-wash steps, no separation steps) assay.

HLA-C (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human HLA-C enables the quantitative determination of human MHC class I antigen C (HLA-C) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

Anti-methyl-Histone H4 Arginine 3 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H4 methylated at arginine 3 (H4R3me). This bead can be used to create AlphaLISA no-wash epigenetic writer and eraser assays.
AlphaPlex Terbium immunoassay kits

α-tubulin (human) AlphaPlex Terbium Detection Kit

The AlphaPlex™ Terbium (Tb) Human α-Tubulin Detection Kit is designed for the quantitative determination of human α-tubulin in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay. This assay can be used as a cell normalization or protein level normalization assay, in conjunction with AlphaLISA® biomarker detection kits.
AlphaLISA immunoassay kits

Insulin (human) AlphaLISA Detection Kit

The AlphaLISA® Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture supernatants, serum, and plasma using a homogeneous (no-wash steps, no separation steps) assay.
AlphaLisa Analyte 2

IL-6 (monkey), lyophilized (AlphaLISA)

Cynomolgus monkey IL-6 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA monkey IL-6 Detection Kit, but can also be ordered separately.

Total Ghrelin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human total ghrelin enables the quantitative determination of total ghrelin in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA SureFire Ultra kits

Phospho-p38 MAPK (Thr180/Tyr182) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Tyr180/182) in cellular lysates using Alpha Technology.

MMP-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human matrix metalloproteinase 23 (MMP-23) enables the quantitative determination of human MMP-23 in buffer, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

Amyloid β 1-40 (human) AlphLISA Detection Kit

The AlphaLISA® immunoassay kit for detection and quantitation of human Amyloid β 1-40 (Aβ 1-40) in cerebrospinal fluid (CSF), cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
AlphaLISA assay principle

Ghrelin (active) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for active human ghrelin enables the quantitative determination of active ghrelin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA Cellular Epigenetics kits

AlphaLISA Human Total Histone H2A Cell-based Assay Kit

AlphaLISA no-wash assay kit for the detection of total human Histone 2A (H2A) in cell extracts or cells in culture medium.
AlphaLISA immunoassay kits

BCL-2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for BCL-2 enables the detection and quantitation of human BCL-2 in serum, buffered solution, or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). This kit is not cross reactive with mouse Bcl-2.

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