PerkinElmer

Alpha试剂

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha试剂" 1-50916 产品与服务

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AlphaPlex-545-antibody-bead2

Anti-FLAG AlphaPlex-545 (Tb) Acceptor beads

AlphaPlex-545 (Terbium) Acceptor beads conjugated to anti-FLAG antibody, for use in Alpha no-wash multiplexing assays.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH Assay Kit

The AlphaLISA® SureFire® Ultra™ Total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for cell number.
SureFire principle

AlphaScreen SureFire S6RP (p-Ser240;Ser244) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH (Human/Mouse) Assay Kit

The AlphaLISA® SureFire® Ultra Mouse/Human GAPDH assay is a sandwich immunoassay for quantitative detection of human or mouse GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SLP-76 (Ser376) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SLP-76 (Ser376) in a multiplexed assay.
AlphaLISA immunoassay kits

CCL5/RANTES (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-cell Expressed and Secreted (CCL5 / RANTES) Detection Kit is designed for detection and quantitation of human CCL5/RANTES in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
AlphaPlex Samarium Acceptor Beads

Anti-Human IgG AlphaPlex 645 (Sm) Acceptor beads

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-human IgG antibody, for use in Alpha no-wash multiplexing assays.
AlphaLISA immunoassay kits

AlphaLISA Human Programmed Cell Death Protein 1 (PD-1) Detection Kit

The AlphaLISA® Human PD-1 Detection Kit is designed for detection and quantitation of Human Programmed Cell Death Protein 1 (PD-1) in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IL2 Detection Kit

The AlphaPlex™ 645 (Samarium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
AlphaLISA assay principle

Osteoprotegerin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for total osteoprotegerin enables the quantitative determination of human osteoprotegerin in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

Galectin-9 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human galectin-9 enables the quantitative determination of human galectin-9 (Gal-9) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA assay principle

IL-6 (monkey) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for monkey IL-6 enables the quantitative determination of cynomolgus monkey interleukin-6 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr421/Ser424) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr421/Ser424) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr421/Ser424) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit
AlphaPlex-545-antibody-bead2

Anti-unmodified H3K4 AlphaPlex-545 Acceptor Beads

AlphaPlex-545 (Terbium) Acceptor beads conjugated to antibody against human Histone H3 that is unmodified (H3K4), for use in Alpha no-wash multiplexing epigenetic assays.

Protein G AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to Protein G. These beads can be used to capture antibodies that associate with Protein G.
AlphaLISA Cellular Epigenetics kits

AlphaLISA Acetylated Histone H3 Lysine 9 (H3K9ac) Cellular Detection Kit

This kit is designed for the detection of acetylated Histone H3 Lysine 9 (H3K9ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
PerkinElmer

Protein A AlphaPlex™ 545 Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to Protein A, for use in Alpha no-wash multiplexing assays.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-MEK1 (Ser218/222) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-MEK1 (Ser218/222) in a multiplexed assay.
AlphaLISA immunoassay kits

Ghrelin (active) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for active human ghrelin enables the quantitative determination of active ghrelin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

EGFR (human) AlphaLISA Detection Kit

The AlphaLISA® EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor (EGFR) in cell culture media, human serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.
AlphaLISA SureFire Ultra kits

Phospho-AMPKα 1/2 (Thr172) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra p-AMPKα 1/2 (Thr172) assay is a sandwich immunoassay for quantitative detection of phospho-AMPKα (phosphorylated on Thr172) in cellular lysates using Alpha Technology.
AlphaLISA immunoassay kits

COL1A1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human COL1A1 enables the detection and quantitation of human COL1A1 in serum, plasma, cell culture supernatants, cell lysates, and tissue homogenates using a homogeneous AlphaLISA assay (no wash steps).
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA immunoassay kits

CYR61 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human cysteine rich angiogenic inducer 61 (CYR61) enables the quantitative determination of human Cyr61 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA assay principle

Total Ghrelin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human total ghrelin enables the quantitative determination of total ghrelin in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA immunoassay kits

IgG4 (human) Pharmacokinetic AlphaLISA Detection Kit

The AlphaLISA® Human IgG4 Pharmacokinetic Detection Kit is designed for the quantitative determination of human IgG4 in monkey serum and other samples, using a homogeneous (no wash steps, no separation steps) assay.

Collagen IV (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human collagen IV enables the quantitative determination of collagen IV in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

D-dimer (human) AlphaLISA Detection Kit

The AlphaLISA® Human D-dimer Detection Kit is designed for detection and quantitation of human D-dimer in citrate plasma, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note the kit shows some cross-reactivity to D-monomer.

CXCL10/IP-10 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse Interferon Gamma Induced Protein 10 (mIP-10)/CXCL10 enables the quantitative determination of mouse IP-10 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SMAD1 (Ser463/465) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD1 (Ser463/465) in a multiplexed assay.
AlphaLisa Analyte 2

OX40L (human), lyophilized (AlphaLISA)

Recombinant human OX40L standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human OX40L detection kit, but can also be ordered separately.
AlphaScreen Acceptor Beads

AlphaScreen Protein A Acceptor beads

AlphaScreen Acceptor beads conjugated to Protein A.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total mTOR Assay Kit

The AlphaLISA® SureFire® Ultra Total mTOR assay is a sandwich immunoassay for quantitative detection of mTOR (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
AlphaLISA SureFire Ultra kits

Phospho-mTOR (Ser2448) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.
AlphaLISA Buffer

5X AlphaLISA Casein Buffer

Casein buffer for prevention of non-specific binding in AlphaLISA no-wash assays. This product is designed to be used in AlphaLISA binding kits. The Casein Buffer was specially developed buffer to reduce the background in AlphaLISA binding assay.
alphascreen surefire total

AlphaScreen SureFire Total IkB alpha Assay Kit

The AlphaScreen® SureFire® total p70 S6K assay is a sandwich immunoassay for quantitative detection of p70 S6K (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total SLP-76 Assay Kit

The AlphaLISA® SureFire® Ultra Total SLP-76 assay is a sandwich immunoassay for quantitative detection of SLP-76 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
SureFire principle

AlphaScreen SureFire Phospho-GSK 3a (Ser21) Kit

The AlphaScreen® SureFire® p-GSK 3α (Ser21) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3α (phosphorylated on Ser21) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-mTOR (Ser2448) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-mTOR (Ser2448) in a multiplexed assay.

ApoB (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ApoB enables the quantitative determination of human apolipoprotein B in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLisa Analyte 2

CCL5/RANTES (mouse), lyophilized (AlphaLISA)

Mouse C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-Cell Expressed, and Secreted (CCL5/RANTES) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse CCL5/RANTES kit, but can be ordered separately.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Insulin Receptor β (Tyr1150/1151) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-Insulin Receptor β (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-INSR (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.

Fc-fragment (human) conjugated AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to human IgG Fc fragment. These beads can be used to capture Fc binding proteins and receptors.
AlphaLISA immunoassay kits

MBNL1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human muscleblind-like protein 1 (MBNL1) enables the quantitative determination of human MBNL1 in buffered solution and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

IL-2 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interleukin-2 (IL-2) Detection Kit is designed for detection and quantitation of mouse IL-2 in serum, buffered solution, or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

IL-15 (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA® Human IL-15 Biotin-Free Detection Kit is designed for the quantitative detection of human Interleukin 15 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.

Lambda light chain (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human lambda light chain enables the quantitative determination of human λ light chain in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-AKT1/2/3 (Thr308) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
AlphaLisa Analyte 2

Laminin (human), lyophilized (AlphaLISA)

Human laminin standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human Laminin Detection Kit, but can be ordered separately.

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