PerkinElmer

AlphaLISA Biomarker Detection Kits

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将麻烦的传统ELISA实验替换成更为优越的选择 -PerkinElmer高度灵敏,均相的生物标志物和细胞因子AlphaLISA检测技术。对于生物标志物,细胞因子和其他分析物检测,我们独有的AlphaLISA分析平台提供了更宽的动态范围和简单的自动化分析选择。从方法开发到初筛再到和复筛,Alpha技术为研究人员提供了适用于多靶点的稳定且易于建立的免疫分析方法。借助AlphaLISA,您还可以将检测时间缩短至少于3小时,并仅消耗少量样本(1-5μL)进而保存珍贵样本。

  • 免洗步骤,无分离步骤
  • ELISA替代技术
  • 高敏感度检测
  • 广泛的样本兼容性
  • 仅消耗极少样本量
  • 结果不到3小时可得到
  • 只需ELISA测定的一半时间

细胞裂解液,血清或血浆样品中的待测物、分别被生物素化抗体和受体微珠偶联的抗体识别,形成三明治形式。之后加入链霉亲和素包被的供体微珠结合生物素化抗体。在680nm激光的激发下,含有高浓度光敏剂的供体微珠被激活并使周围环境中的氧分子转化成高能活跃的单体氧(singlet oxygen),在4微秒的半衰期内,单体氧在溶液中扩散触发受体微珠上的化学分子,并瞬间激发一系列化学反应后最终使受体微珠产生615nm的光信号,产生的光信号与被检测物的浓度成正相关。

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"AlphaLISA Biomarker Detection Kits" 1-50291 产品与服务

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AlphaLISA immunoassay kits

IgG2a (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG2a antibodies in cell culture media and non-mouse serum.
AlphaLISA assay principle

IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-23 enables the quantitative determination of human interleukin-23 in human serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA assay principle

ANGPT1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ANGPT1 enables the quantitative determination of human Angiopoietin 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

IFN-λ2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human interferon lambda-2 (IFN-λ2) enables the quantitative determination of human IFN-λ2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA assay principle

Cardiac Troponin T (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human cardiac troponin T enables the quantitative determination of cardiac troponin T in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

Vimentin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human vimentin enables the quantitative determination of human vimentin in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

HIV p24 AlphaLISA Biotin-Free Detection Kit

The AlphaLISA HIV p24 Biotin-Free Detection Kit is designed for the quantitative detection of HIV p24 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
AlphaLISA immunoassay kits

IgG2b (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG2b antibodies in cell culture media and non-mouse serum.
AlphaLISA immunoassay kits

Ghrelin (active) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for active human ghrelin enables the quantitative determination of active ghrelin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

Aflatoxin AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for aflatoxin enables the quantitative determination of aflatoxin in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

ß-NGF (human) AlphaLISA Detection Kit

The AlphaLISA® Human Beta-Nerve Growth Factor (β-NGF) Detection Kit is designed for detection and quantitation of human β-NGF in cell culture medium and serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

TIMP-2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human tissue inhibitor of metalloproteinases-2 (TIMP-2) enables the quantitative determination of human TIMP-2 in buffered solution, serum, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).

PTEN (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PTEN enables the detection and quantitation of human PTEN in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps). Other species of PTEN were not tested, but detection is expected with mouse and rat PTEN due to >99% sequence homology.
AlphaLISA immunoassay kits

Chloramphenicol Biotin-free AlphaLISA Detection Kit

The AlphaLISA® biotin-free immunoassay kit for chloramphenicol enables the quantitative determination of chloramphenicol in buffered solution, milk and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The kit utilizes a Digoxigenin (DIG) / Anti-DIG interaction as opposed to the traditional Streptavidin/Biotin interaction. This enables optimal performance when working with biotin-rich media (e.g. RPMI) or samples containing endogenous biotin.

FLT-1/VEGFR 1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human VEGFR 1 (FLT-1) enables the quantitative determination of VEGFR 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

Laminin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human laminin enables the detection and quantitation of human laminin in human serum, plasma, lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL-28A, IL-29, and Mouse Laminin.
AlphaLISA immunoassay kits

Aβ 1-40 (human) High Specificity AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human Aβ40 enables the detection and quantitation of human Aβ40 in human cerebrospinal fluid and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with other forms of Amyloid beta (i.e. Aβ1-38 and Aβ1-42).

Beta-2 Microglobulin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human beta-2 microglobulin enables the quantitative determination of human beta-2 microglobulin in buffered solution, serum, CSF, urine, saliva, cell culture supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

IL-25 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-25 enables the quantitative determination of mouse interleukin-25 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

Perlecan (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human perlecan enables the detection and quantitation of human perlecan (endorepellin) in human serum and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay showed no cross reacitivity to vimentin, fibronectin, nidogen-1, laminin, or collagen I.

CCL5/RANTES (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-cell Expressed and Secreted (CCL5 / RANTES) Detection Kit is designed for detection and quantitation of human CCL5/RANTES in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

IgM (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgM in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
AlphaLISA immunoassay kits

IL-17F (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for IL17F is designed for the quantitative determination of human IL17F in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL-17A and with mouse IL17F, bovine IL17F, and rat IL17F. Cross-reactivity with other species has not tested.

Frataxin (human) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of human Frataxin (hFXN) in cell and tissue lysates.

PER.C6® HCP AlphaLISA Detection Kit

The AlphaLISA® PER.C6® HCP Detection Kit is designed for the quantitative detection of PER.C6® contaminants in buffer and cell culture media using a homogeneous (no wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

IFN-α (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon-alpha (IFN-α) Detection Kit is designed for detection and quantitation of human IFN-α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit consists of the IFN-α 2b subtype.
AlphaLISA immunoassay kits

IgA (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgA in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
AlphaLISA immunoassay kits

ApoA1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoA1 is designed for the quantitative determination of ApoA1 in human serum, human plasma and cell supernantants using a homogeneous AlphaLISA assay (no wash steps). Neglible cross reactivity was observed with ApoCIII, ApoE, or ApoB.

IgE (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgE antibodies in different sample matrices, including cell culture media. The IgE subtype plays a role in allergic reactions, parasitic infections and hypersensitivity reactions.

CD28 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD28 enables the quantitative determination of human CD28 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

IP-10 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.

PIP Collagen (human) AlphaLISA Detection Kit

The AlphaLISA® Human PIP Detection Kit is designed for detection and quantitation of Human Procollagen Type I C-peptide (PIP) in human serum, plasma, cell culture supernatants, cell lysate, and tissue homogenates using a homogeneous (no-wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

FSH (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human FSH enables the quantitative determination of human follicle stimulating hormone in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

Glucagon (human) AlphaLISA Detection Kit

The AlphaLISA® Glucagon Detection Kit is designed for detection and quantitation of glucagon in cell culture media, sera and plasma, in a homogeneous (no-wash steps, no separation steps) assay.

IGF-1 (rat) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for rat IGF-1 enables the quantitative determination of rat insulin-like growth factor 1 (IGF-1) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

TSLP (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human TSLP enables the quantitative determination of human TSLP in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

Factor VIII (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLaISA® Human Factor VIII Biotin-Free Detection Kit is designed for the quantitative detection of human Factor VIII in buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
AlphaLISA immunoassay kits

Galectin-9 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human galectin-9 enables the quantitative determination of human galectin-9 (Gal-9) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

IFN-γ (cynomolgus) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for monkey cynomolgus interferon gamma (IFNγ) enables the quantitative determination of cynomolgus IFNγ in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

ApoE (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoE enables the quantitative determination of Apolipopriein E (ApoE) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimum cross-reactivity with ApoA1, ApoB, and ApoCIII. Crossreactivity with other species has not tested.
AlphaLISA immunoassay kits

IFNα (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for IFNα is designed for the quantitative determination of mouse interferon-alpha (IFN-α) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

IgG2 (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgG2 in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
AlphaLISA immunoassay kits

BMP-7 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human BMP-7 enables the detection and quantitation of human BMP-7 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

IL-13 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for IL13 is designed for the quantitative determination of IL13 in porcine serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human IL13.
AlphaLISA immunoassay kits

OX40L (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human OX40L enables the quantitative determination of human OX40 ligand in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

HLA-E (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human HLA-E enables the quantitative determination of human MHC class I antigen E (HLA-E) in buffered solution, cell culture supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

ApoB (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ApoB enables the quantitative determination of human apolipoprotein B in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

IL-2 (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-2 enables the detection and quantitation of bovine interleukin-2 in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

CD117 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD117 enables the quantitative determination of human CD117 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

IL-11 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin 11 (IL-11) Detection Kit is designed for detection and quantitation of IL-11 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

"AlphaLISA Biomarker Detection Kits" 1-50291 产品与服务

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