PerkinElmer

细胞信号和途径分析

OF

从受体配体结合到磷酸化蛋白的细胞内通路分析,实验室获得了细胞信号传导和信号转导的突破性发现,提高结果质量并简化了相关流程。

我们凭借完整细胞水平生化分析解决方案,获得更深入的理解以及更具生物学相关性的结果,如以下创新试剂技术:AlphaLISA SureFire Ultra、AlphaLISA SureFire Ultra Multiplex、AlphaScreen SureFire、LANCE Ultra TR-FRET和DELFIA TRF试验——以及我们整套行业领先的放射化学品系列。

凭借一系列高可靠性、高性能的全波长酶标仪:包括:EnVision和EnSight系统,我公司提供了多种检测技术,满足您实现下一个重大突破的一切需求。

Cellular Kinase Assays
Cellular Kinase Assays

细胞激酶检测

AlphaSureFire®检测属于免洗涤细胞激酶检测。虽然有着不同的形式(AlphaScreen®SureFire,AlphaLISA®SureFireUltra和Alpha SureFire Ultra Multiplex),但均采用珀金埃尔默独有的微珠技术,通过夹心法免疫反应方式,检测细胞中的磷酸化蛋白质。Alpha SureFire检测是蛋白免疫印迹法(Western Blotting)的定量性替代方法,可实现自动化、微型化,并能够检测内源性和重组蛋白。

AlphaScreen SureFire

AlphaScreen SureFire拥有大量小分子筛选的成功案例记录:

  • 用蛋白A捕获其中一个试剂盒抗体
  • 检测时间4小时
  • 除AlphaScreen SureFire试剂盒外,还需订购AlphaScreen蛋白A试剂盒

AlphaLISA SureFire Ultra

用CaptSure™(小肽标记)标记的其中一种抗体,能被CaptSure-抗体微珠识别,与AlphaScreen SureFire方式相比具有显着改进:

  • 本底信号较低、灵敏度更高和动态范围更大
  • 不受外来抗体的干扰;非常适用于治疗性抗体筛查
  • AlphaLISA微珠是含血红蛋白样本的最佳选择
  • 检测速度快:检测时间仅需2小时

Alpha SureFire Ultra Multiplex

本形态应用AlphaPlex™技术读取来自同一板孔的多个信号:

  • 通过内部控制提高了数据质量(例如:总蛋白),或能够从单个实验中获得更多数据,从而大幅节约了时间和节省了成本。
  • 与AlphaLISA SureFire Ultra特性相同,试剂盒中含有微珠
GPCR Studies
GPCR Studies

G蛋白偶联受体研究

我们提供多种经验证G蛋白偶联受体靶标解决方案——从配体G蛋白偶联受体到细胞激活信号转导级联的实际评估。确保您能够进行微珠确定、验证、筛选和配置。

LANCE Ultra时间分辨荧光共振能量转移

LANCE® Ultra TR-FRET技术可为研究人员提供高质量操作简单的试验,满足多模式探测器各类标靶的测定要求,市场上可购买TR-FRET试验,试验时间短。

  • 免洗涤/无分离步骤
  • 均相分配开盒即用技术
  • 灵敏检测
  • 重现性高
  • 测定时间短
  • 便于自动化
  • 384孔板和1536孔板

LANCE和LANCE(镧系元素螯合物激发)Ultra是TR-FRET(时间分辨荧光共振能量转移)和均相(无需洗涤)技术。其中一个结合配体标有供体荧光团(LANCE铕螯合物),另一个标有受体荧光团(ULight™受体染料)。在320-340nm激发后,如果距离满足荧光共振能量转移要求(~10nm),则能量可以从供体铕螯合物转移到受体荧光团上。因此可在665nm处探测到光线发射。

DELFIA

DELFIA(解离增强镧系元素荧光免疫分析平台)是一种经验证的基于时间分辨荧光检测的强大免疫检测平台。这种检测方法与镧系元素螯合物的独特化学性质相结合,使DELFIA成为传统ELISA在研究、药物研发和临床应用领域的改良代替品。

DELFIA能够实现传统ELISA无法实现的高灵敏度、宽动态范围和持久的稳定性。

辐射检测

辐射检测是一种直接的检测方法,其无需标靶特异性抗体,并能够缩短检测时间,减少变量。一系列的同位素(3H、14C、33P、35S)可被标记为多个品类而不改变其生物学功能,以便研究最天然状态下的受体配体结合、激酶、G蛋白和其他细胞信号通路。

对于高通量筛选,SPA(闪烁迫近分析法)技术能够为多种检测提供同质辐射测量形式。

  • 无分离和洗涤步骤,节约时间,结果可靠。
  • 多种SPA微珠染料可测定多种标靶。
  • 操作方便、性价比高、可代替放射性过滤器绑定膜过滤结合分析法,与之相比更安全。
  • 可自动化以提高通量。
  • SPA微珠可用于管板、96孔、384孔或1536孔微孔板中,并且用任何闪烁计数器测量其信号。
  • 可将检验小型化,减少每孔的总测定体积和浓度,从而降低试剂成本获得更高通量。
  • SPA微珠适用于3H-、125I-、33P-、35S-和14C-标记的配体。
  • 无需进行蛋白质改性。

另一种形式——SPA Imaging Beads设计用于CCD的微孔板成像仪检测,与SPA Scintillation Beads相比,其优势如下:

  • 红移信号可最大限度地减少有色化合物的猝灭,从而减少漏报数量。
  • 任何板密度的不限方式成像均能够提高通量,从而更快获得试验结果。
EnSight Multimode Plate Reader
EnSight Multimode Plate Reader

全波长酶标仪

我们提供多种波长的酶标仪,以满足所有细胞信号实验室的不同应用要求。每个全波长酶标仪均采用领先技术,因此无论您选择哪种仪器,均可在您特定检验中发挥优异的性能。
  • VICTOR X 全波长酶标仪——多功能的可靠系统,在单个高性价比的平台上提供多种检测技术,提高各种规模的细胞信号传导研究实验室的灵活性、灵敏度并简化操作流程。
  • EnSpire®全波长酶标仪——其设计满足各种用户研究环境的需求,为各类应用提供高性能技术和灵活性,且不影响灵敏度。
  • EnSpire Alpha酶标仪——经验证的Alpha技术,价格较低
  • EnSight™全波长酶标仪——首个能够提供良好成像以及标记和无标记技术的台式系统。EnSight™系统配置独特的检测模式,能够监控细胞样品质量,采用正交方法进行检测。
  • EnVision®多标记酶标仪——市场上检测速率最快的酶标仪之一。EnVision®的多标记系统中有两个探测器,可实现同步、快速双波长读取、高速灵敏的激光TRF和Alpha技术检测。该仪器可扩展,满足您通量和应用需求改变或增长需求。
  • ViewLux®——顶级微孔板成像仪,适用于需要超高速、可靠和高质量数据的化合物库的超高通量筛选。
  • MicroBeta2系统为G蛋白偶联受体、激酶、报告基因检验和传统的液体闪烁计数等应用提供先进的辐射和发光检测能力。拥有符合计数功能,采用独有的专利结构,配有两个同步进行信号检测的光电倍增管,该系统能够在超低本底条件下进行高效放射性核素检验。
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产品类型
  • Reagents (177)
  • Instruments (2)
应用
  • Protein Analysis & Detection (152)
  • Kinase (149)
  • Multiplexing (31)
  • cAMP (3)
检测点(200-50,000)
  • 10000 (151)
  • 500 (148)
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  • 1000 (1)
检测目标
  • Akt1/2/3 (7)
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  • ERK1/2 (7)
  • STAT3 (6)
  • NFκB (6)
  • p70 S6K (6)
  • EGFR (5)
  • p38 MAPK (5)
  • STAT5 (5)
  • STAT1 (5)
  • SLP-76 (4)
  • Cofilin (4)
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  • GAPDH (2)
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  • BAD (2)
  • AKT1/2/3 (2)
  • BTK (2)
  • AMPKα (2)
  • CREB (2)
  • SYK (2)
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  • c-Met (2)
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  • VEGFR2 (2)
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  • JNK1/2/3 (2)
  • TrkA (1)
  • GSK3α (1)
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  • Akt1/2 (1)
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  • Caspase-9 (1)
  • LCK (1)
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  • ATF2 (1)
  • Acetyl CoA carboxylase (1)
  • JNK1/3 (1)
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  • STAT6 (1)
检测目标类
  • Phosphoprotein (137)
  • Protein (33)
自动化兼容
  • Yes (176)
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  • Microplates (94)
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  • Phosphorylation (52)
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  • AlphaScreen SureFire (62)
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  • LANCE (1)
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  • EnVision (1)
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治疗领域
  • Autophagy (1)

"细胞信号和途径分析" 1-50179 产品与服务

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EnSight Multimode Plate Reader

EnSight 多模式读板仪

EnSight 多模式读板仪是一台整合了多标记、无标记和显微成像技术的桌面式一体化检测平台。其整孔成像&在线数据分析速度非常快,可以在五分钟内完成384孔板整板的图像获取及分析。该仪器采用预置模块化设计,结合基于Kaleido软件的数据采集和分析流程,是一台真正的全能且高产的多模式读板仪。
EnVision 2105 multimode plate reader

EnVision 多模式读板仪

EnVision 多模式读板仪在所有检测模式下都能提供最快的检测速度、超高通量及最高的灵敏度,为您的高通量筛选提供可靠的仪器及结果,以满足您日益加速的新实验需求。
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-p38 MAPK (Thr180/Tyr182) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p38 MAPK (Thr180/Tyr182) in a multiplexed assay.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-SLP-76 (Ser376) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-SLP-76 (Ser376) kits are designed for the detection of SLP76 (phosphorylated at S376) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-NF-κB p65 (Ser536) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-NF-κB p65 (Ser536) in a multiplexed assay.
SureFire principle

AlphaScreen SureFire S6RP (p-Ser240;Ser244) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-eIF2A (Ser51) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-eIF2A (Ser51) kits are designed for the detection of eIF2A (phosphorylated at S51) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-SMAD1 (Ser463/465) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-SMAD1 (Ser463/465) kits are designed for the detection of SMAD1 (phosphorylated at S463/465) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-VEGF Receptor 2 (Tyr1175) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-VEGF Receptor 2 (Tyr1175) in a multiplexed assay.
SureFire principle

AlphaScreen SureFire STAT5 (p-Tyr694;Tyr699) Assay Kit 

The AlphaScreen® SureFire® p-STAT5 (p-Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-CREB (Ser133) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-Insulin Receptor β (Tyr1150/1151) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-Insulin Receptor β (Tyr1150/1151) in a multiplexed assay.
AlphaScreen cAMP assay principle

AlphaScreen cAMP Detection Kit

AlphaScreen no-wash assay kit for quantitative detection of cAMP.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
SureFire principle

AlphaScreen SureFire Phospho-MEK 1 Kit

The AlphaScreen® SureFire® p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
SureFire principle

AlphaScreen SureFire Akt 1/2 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt 1/2 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt 1/2 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
alphascreen surefire total

AlphaScreen SureFire Total Topo Iia Assay Kit

The AlphaScreen® SureFire® total Topoisomerase II-α assay is a sandwich immunoassay for quantitative detection of Topoisomerase II-α (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total AKT1 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total AKT1 kits are designed for the detection of total AKT1 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-AKT1, and is compatible with both adherent and suspension cells.
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total SLP-76 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total SLP-76 kits are designed for the detection of SLP-76 (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-SLP-76, and is compatible with both adherent and suspension cells.
AlphaLISA SureFire Ultra kits

Phospho-Syk (Tyr352) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-Syk assay is a sandwich immunoassay for quantitative detection of Syk (phosphorylated on Tyr352) in cellular lysates using Alpha Technology.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SYK (Tyr525/526) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SYK (Tyr525/526) in a multiplexed assay.
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser136) Kit

The AlphaScreen® SureFire® p-BAD (Ser136) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser136) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

Phospho-AMPKα 1/2 (Thr172) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra p-AMPKα 1/2 (Thr172) assay is a sandwich immunoassay for quantitative detection of phospho-AMPKα (phosphorylated on Thr172) in cellular lysates using Alpha Technology.
SureFire principle

AlphaScreen SureFire Phospho-GSK 3b (Ser9) Kit

The AlphaScreen® SureFire® p-GSK 3β (Ser9) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3β (phosphorylated on Ser9) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr421/Ser424) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr421/Ser424) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr421/Ser424) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit
AlphaLISA SureFire Ultra kits

Phospho-Cofilin (Ser3) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology.
SureFire principle

AlphaScreen SureFire EGFR (p-Tyr1068) Assay Kit

The AlphaScreen® SureFire® p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGFR (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-IKKα (Ser176/180) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-IKKα (Ser176/180) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF4E (Ser209) Assay Kits

The AlphaLISA® SureFire® Ultra™ p-eIF4E(Ser209) assay is a sandwich immunoassay for quantitative detection of phospho-eIF4E (phosphorylated on Ser209) in cellular lysates using Alpha Technology.
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr389) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

Total VEGFR-2 AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ Total VEGFR-2 assay is a sandwich immunoassay for quantitative detection of VEGF receptor 2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1586) Assay Kit

The AlphaScreen® SureFire® p-ALK (Tyr1586) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1586) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

Phospho-SMAD2 (Ser465/467) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-SMAD2 assay is a sandwich immunoassay for quantitative detection of SMAD2 (phosphorylated on Ser465/467) in cellular lysates using Alpha Technology.
alphascreen surefire total

AlphaScreen SureFire ALK Total Assay Kit

The AlphaScreen® SureFire® ALK (total) assay is a sandwich immunoassay for quantitative detection of ALK in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing ALK levels for experiments measuring changes in ALK phosphorylation. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total eIF2α LANCE Ultra TR-FRET Detection Kit

LANCE Ultra Total eIF2α kits are designed for the detection of total eIF2α (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-eIF2α detection, and is compatible with both adherent and suspension cells.
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr70) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr70) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr70) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-Src (Tyr419) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-Src (Tyr419) kits are designed for the detection of Src (phosphorylated at residue Y419) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-ERK1/2 (Thr202/Tyr204) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-ERK1/2 (Thr202/Tyr204) in a multiplexed assay.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-CREB (Ser133) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-CREB (Ser133) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

Phospho-Acetyl CoA Carboxylase (Ser79) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra p-ACC (Ser79) assay is a sandwich immunoassay for quantitative detection of phospho-acetyl CoA carboxylase (phosphorylated on Ser79) in cellular lysates using Alpha Technology.
AlphaLISA SureFire Ultra kits

Total STAT3 AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-GSK-3β (Ser9) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-GSK-3β (Ser9) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total ERK 1/2 Assay Kit

The AlphaLISA® SureFire® Ultra™ Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
SureFire principle

AlphaScreen SureFire Phospho-GSK 3a (Ser21) Kit

The AlphaScreen® SureFire® p-GSK 3α (Ser21) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3α (phosphorylated on Ser21) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
SureFire principle

AlphaScreen SureFire STAT3 (p-Tyr705) Assay Kit

The AlphaScreen® SureFire® p-STAT3 (p-Ser705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Ser705) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-ERK 1/2 (Thr202/Tyr204) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-ERK 1/2 (Thr202/Tyr204) kits are designed for the detection of phosphorylated ERK 1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
AlphaLISA SureFire Ultra kits

Phospho-STAT1 (Tyr701) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.

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