PerkinElmer

药物开发试剂

OF

Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE®, DELFIA®, and other assay technologies.

Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET检测

LANCE Ultra技术可为研究人员提供从试验立项到初次和二次筛选的高质量操作简单的试验,满足多模式探测器各类靶标的试验要求,市场上可购买TR-FRET试验,试验时间短。
  • 免洗涤/无分离步骤
  • 均相分配开盒即用技术
  • 灵敏检测
  • 重现性高
  • 测定时间短
  • 便于自动化
  • 384孔板和1536孔板
LANCE和LANCE(镧系元素螯合物激发)Ultra是TR-FRET(时间分辨荧光共振能量转移)和均相(无需洗涤)技术。其中一个结合配体标有供体荧光团(LANCE铕螯合物),另一个标有受体荧光团(ULight™受体染料)。激发到320-340nm时,如果距离范围满足荧光共振能量转移要求(~10 nm),则能量可从供体螯合物转移到受体荧光团上。因此可在665nm处探测到光线发射。
DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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"药物开发试剂" 1-501374 产品与服务

每页

TGF-β1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human TGF-β1 Detection Kit is designed for detection and quantitation of human TGF-β1 (hTGF-β1) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with human hTGF-β2 and hTGF-β3.
AlphaLISA immunoassay kits

CXCL13 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CXCL13 enables the quantitative determination of human C-X-C Motif Chemokine Ligand 13 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).

Neprilysin (human) AlphaLISA Detection Kit

The AlphaLISA® Neprilysin Detection Kit is designed for detection and quantitation of human neprilysin (NEP, CD10) in cell culture media, serum, and CSF (cerebrospinal fluid) in a homogeneous (no-wash steps, no separation steps) assay.

LANCE Ultra ULight™-phospho-40S ribosomal protein S6 (pSer235/236) Peptide

Synthetic peptide containing residues surrounding Ser235 and Ser236 of human 40S ribosomal protein S6; this peptide is pre-phosphorylated on Ser235; phosphorylation site: Ser236. This peptide is phosphorylated at Ser236 efficiently by kinases from the RSK family. Phosphorylation motif: RRL(pS)SLRA
AlphaLISA assay principle

Cardiac Troponin T (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human cardiac troponin T enables the quantitative determination of cardiac troponin T in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

COL1A1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human COL1A1 enables the detection and quantitation of human COL1A1 in serum, plasma, cell culture supernatants, cell lysates, and tissue homogenates using a homogeneous AlphaLISA assay (no wash steps).

Anti-Mouse IgG1 (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG1. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.

IGF-1 (rat) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Rat IGF-1 Detection Kit is designed for detection and quantitation of rat insulin-like growth factor 1 (IGF-1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

Host Cell Residual DNA AlphaLISA Detection Kit

The AlphaLISA® Host Cell Residual DNA Detection Kit is designed for detection and quantitation of DNA contaminants in cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay. This kit can be used with DNA from different prokaryotic and eukaryotic species.
SureFire principle

AlphaScreen SureFire Phospho-cofilin (Ser3) Assay Kit

The AlphaScreen® SureFire® p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-Cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.

Trefoil factor 3 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Trefoil Factor 3 (TFF3) Detection Kit is designed for detection and quantitation of human TFF3 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

Anti-Maltose-Binding Protein (MBP) AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-MBP (maltose-binding protein) antibody. The antibody is a rat monoclonal antibody, clone MBP 7G4. These beads can be used to capture MBP-tagged proteins for AlphaLISA no-wash assays.
AlphaLISA immunoassay kits

Cortisol (human) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection of human cortisol in buffer or serum.

LANCE Ultra Europium-anti-phospho-Acetyl-CoA Carboxylase (Ser79) Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from rat Acetyl-CoA Carboxylase phosphorylated at Ser79. Acetyl-CoA Carboxylase peptides can be phosphorylated by the AMPKα1 kinase. Sequence motif recognized: SSM-pS-GL
AlphaLisa Analyte 2

CCL2 / MCP1 (human), lyophilized (AlphaLISA)

Human Monocyte Chemoattractant Protein-1 (CCL2 / MCP1) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human CCL2/MCP1 kit, but can be ordered separately.

MMP-7 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human MMP7 enables the detection and quantitation of human pro- and active MMP-7 (hMMP-7) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

Alpha-synuclein (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human Alpha-synuclein enables the quantitative determination of Alpha-synuclein in biological fluids such as serum, plasma, cerebrospinal fluid and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
LANCE Detection Kit

Leptin (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse Leptin Detection Kit is designed for detection and quantitation of mouse leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

GDNF (human) AlphaLISA Detection kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of human GDNF (glial cell-derived neurotrophic factor) in serum, plasma, cell culture supernatants, and other samples.
SureFire principle

AlphaScreen SureFire CHK2 (p-Thr68) Assay Kit 

The AlphaScreen® SureFire® p-CHK2 (Thr68) assay is a sandwich immunoassay for quantitative detection of phospho-CHK2 (phosphorylated on Thr68 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

Phospho-p38 MAPK (Thr180/Tyr182) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Tyr180/182) in cellular lysates using Alpha Technology.

IL-12/IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-12 enables the detection and quantitation of human Interleukin 12 p40 (IL12 p40) in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). It is important to note that this assay will also detect IL-23 p40.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total NFκB LANCE Ultra TR-FRET Detection Kit

LANCE Ultra total NFκB kits are designed for the detection of NFκB (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-NFκB assay.

IFN-β (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon β (IFNβ) Detection Kit is designed for detection and quantitation of human IFNβ in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit corresponds to the IFN-ß 1a subtype.
Alpha donor antibody coated

Anti-GFP Alpha Donor Beads

Alpha Donor beads conjugated to anti-GFP antibody. The antibody is a mouse monoclonal antibody that recognizes wildtype and recombinant GFP (rGFP), and cross-reacts with eGFP. These beads can be used to capture GFP-tagged proteins for AlphaScreen, AlphaLISA, and AlphaPlex no-wash assays.

PDGF (BB dimer) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PDGF (BB homodimer) enables the quantitative determination of human platelet-derived growth factor (PDGF) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

AlphaLISA anti-mouse IgG Acceptor beads

AlphaLISA Acceptor beads conjugated to anti-mouse IgG antibody. This antibody is not Fc-specific; for Fc-specific beads, please see product AL105.
AlphaLisa Analyte 2

IL-2 (human), lyophilized (AlphaLISA)

Human Interleukin-2 (IL2) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL-2 kit, but can be ordered separately.

LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36)

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA® Eu-N1 Anti-GST

Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in DELFIA® TRF assays.
AlphaLISA SureFire Ultra kits

Total VEGFR-2 AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ Total VEGFR-2 assay is a sandwich immunoassay for quantitative detection of VEGF receptor 2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Protein

Osteoprotegerin (human), lyophilized (AlphaLISA)

Human osteoprotegerin standard, for use in running standard curves in AlphaLISA assays.

LANCE Ultra ULight™-anti-GST

Goat polyclonal anti-GST tag antibody labeled with ULight™ dye.
AlphaLISA immunoassay kits

IL-8 (human) AlphaLISA Detection Kit

The AlphaLISA Human IL8 Detection Kit is designed for the quantitative determination of human IL-8 in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

CD86 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD86 enables the quantitative determination of human CD86 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Alpha_Donor_bead_1

AlphaScreen Streptavidin Donor beads

Alpha Donor beads conjugated to streptavidin.
AlphaLISA immunoassay kits

NGAL (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for NGAL is designed for the quantitative determination of Neutrophil Gelatinase Associated Lipocalin (NGAL) also known as lipocalin 2 in cell culture media and human serum using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse NGAL, rat NGAL, bovine NGAL and chicken NGAL.
AlphaLisa Analyte 2

NT-proBNP (human), lyophilized (AlphaLISA)

Recombinant human N-terminal prohormone of brain natriuretic peptide (NT-proBNP) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human NT-proBNP detection kit, but can also be ordered separately.
LANCE Detection Kit

IgG (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse IgG Detection Kit is designed for detection and quantitation of Mouse IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

DELFIA Eu-Labeling kit (Eu-N1-ITC chelate), 0.2mg

DELFIA® Europium labeling kit for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
AlphaLISA immunoassay kits

ApoA1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoA1 is designed for the quantitative determination of ApoA1 in human serum, human plasma and cell supernantants using a homogeneous AlphaLISA assay (no wash steps). Neglible cross reactivity was observed with ApoCIII, ApoE, or ApoB.
LANCE Detection Kit

BDNF (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human BDNF Detection Kit is designed for detection and quantitation of human BDNF in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Anti-goat IgG (Fc specific) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-goat IgG antibody. These beads can be used to capture goat IgG antibodies. The antibody is Fc-specific, and is a rabbit polyclonal F(ab')2 fragment.
AlphaLISA immunoassay kits

TNFα (mouse) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Mouse TNFα Biotin-Free Detection Kit is designed for the quantitative detection of mouse TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
AlphaLISA immunoassay kits

GAD67 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for GAD67 enables the quantitative determination of human glutamate decarboxylase 67 (hGAD67) in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows less than 5% cross reactivity with mouse GAD67. Cross-reactivity with other species has not tested.
AlphaLisa Analyte 2

hCG (human), lyophilized (AlphaLISA)

Human chorionic gonadotropin (hCG) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA hCG Detection Kit, but can also be ordered separately.
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA immunoassay kits

CXCL1/GROα (human) AlphaLISA Detection Kit

The AlphaLISA® Human CXCL1/GROα Detection Kit is designed for detection and quantitation of Human CXCL1/GROα in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
AlphaLISA SureFire Ultra kits

Phospho-GSK-3β (Ser9) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-GSK-3β (Ser9) assay is a sandwich immunoassay for quantitative detection of endogenous GSK-3β phosphorylated at Ser9 in cellular lysates using Alpha Technology.

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