PerkinElmer

药物开发试剂

OF

Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE®, DELFIA®, and other assay technologies.

Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET检测

LANCE Ultra技术可为研究人员提供从试验立项到初次和二次筛选的高质量操作简单的试验,满足多模式探测器各类靶标的试验要求,市场上可购买TR-FRET试验,试验时间短。
  • 免洗涤/无分离步骤
  • 均相分配开盒即用技术
  • 灵敏检测
  • 重现性高
  • 测定时间短
  • 便于自动化
  • 384孔板和1536孔板
LANCE和LANCE(镧系元素螯合物激发)Ultra是TR-FRET(时间分辨荧光共振能量转移)和均相(无需洗涤)技术。其中一个结合配体标有供体荧光团(LANCE铕螯合物),另一个标有受体荧光团(ULight™受体染料)。激发到320-340nm时,如果距离范围满足荧光共振能量转移要求(~10 nm),则能量可从供体螯合物转移到受体荧光团上。因此可在665nm处探测到光线发射。
DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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"药物开发试剂" 1-501358 产品与服务

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AlphaLISA immunoassay kits

IL-18BP (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-18BP enables the quantitative determination of human interleukin 18-binding protein in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

IgG (mouse) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgG in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
AlphaLisa Analyte 2

PD-L2 (human), lyophilized (AlphaLISA)

Recombinant human PD-L2 standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human PD-L2 detection kit, but can also be ordered separately.
AlphaLISA immunoassay kits

IL-10 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine IL-10 enables the quantitative determination of porcine interleukin 10 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLisa Analyte 2

IgA (mouse), lyophilized (AlphaLISA)

Mouse IgA standard, 0.1 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA mouse IgA kits, but can also be ordered separately.

Beta-2 Microglobulin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human beta-2 microglobulin enables the quantitative determination of human beta-2 microglobulin in buffered solution, serum, CSF, urine, saliva, cell culture supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SMAD1 (Ser463/465) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD1 (Ser463/465) in a multiplexed assay.

LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
AlphaLISA immunoassay kits

IL-33 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-33 enables the quantitative determination of mouse interleukin-33 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA assay principle

IL-31 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for IL-31 enables the quantitative determination of human IL-31 in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse IL31. Cross-reactivity with other species has not been tested.

IL-1α (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-1α Detection Kit is designed for detection and quantitation of human interleuukin-1α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser112) Kit

The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLisa Analyte 2

IL-2 (bovine), lyophilized (AlphaLISA)

Bovine IL-2 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Bovine IL-2 Detection Kit, but can be ordered separately.

Biotin-anti-H3K79me2 antibody

Biotinylated antibody against human histone H3 that is dimethylated on Lysine 79. This antibody is intended for use in AlphaLISA® no-wash epigenetic assays using DOT1L methyltransferase with oligonucleosome substrates.
AlphaLISA immunoassay kits

CCL1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CCL1 enables the quantitative determination of human chemokine (C-C motif) ligand 1 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT1 (Ser727) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Ser727) in a multiplexed assay.

LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36)

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM

p62 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for p62 enables the quantitative determination of p62 in human cell lysates using a homogeneous AlphaLISA assay (no wash steps).

Anti-GFP AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-GFP antibody. The antibody is a mouse monoclonal antibody that recognizes wildtype and recombinant GFP (rGFP), and cross-reacts with eGFP. These beads can be used to capture GFP-tagged proteins for AlphaLISA no-wash assays.
AlphaLISA immunoassay kits

IL-25 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-25 enables the quantitative determination of mouse interleukin-25 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

Vimentin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human vimentin enables the quantitative determination of human vimentin in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).

IgA (mouse) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgA in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.

AlphaLISA Biotin-anti-Histone H4 Antibody

AlphaLISA® biotinylated monoclonal antibody recognizing the amino-terminal end of human histone H4. Broad species cross-reactivity is expected based on sequence similarity. This product is designed to be used with AlphaLISA® Epigenetics Acceptor beads to detect modified full-length histone H4 in homogeneous AlphaLISA assays.
AlphaLISA binding assay

PDGF-BB/PDGFRα (human) AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRα, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRα.
AlphaLisa Analyte 2

Troponin I (human), lyophilized (AlphaLISA)

Human Troponin I, lyophilized, for use as a standard for running standard curves in AlphaLISA no-wash immunoassays. This standard is already included in AlphaLISA Troponin kits, but can be ordered separately.
LANCE Detection Kit

CXCL1/GRO-α (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human CXCL1/GRO-α Detection Kit is designed for detection and quantitation of human CXCL1 (GRO-α) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

Laminin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human laminin enables the detection and quantitation of human laminin in human serum, plasma, lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL-28A, IL-29, and Mouse Laminin.
AlphaLISA immunoassay kits

CHO HCP (broad reactivity) AlphaLISA Detection Kit

AlphaLISA® no-wash immunoassay kit for detection of Chinese Hamster Ovary Host Cell Proteins (CHO HCP) in buffered solution or cell culture medium.
AlphaLISA immunoassay kits

IL-17A (mouse/rat) AlphaLISA Biotin-free Detection Kit

The AlphaLISA Mouse/Rat IL-17A Biotin-Free Detection Kit is designed for the quantitative detection of mouse or rat IL-17 (IL-17A) in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
alphaimmunoassay1

IL-1β (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin-1β (IL1β) Detection Kit is designed for detection and quantitation of human IL-1β in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLisa Analyte 2

p24 (human HIV), lyophilized (AlphaLISA)

Human Immunodeficiency Virus type-1 p24 protein (HIV p24) standard, lyophilized, for use in running standard curves in AlphaLISA® no-wash immunoassays. This standard is already provided in the AlphaLISA HIV p24 kit, but can be ordered separately.

Strep-Tactin® AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to Strep-Tactin®. These beads can be used to capture Strep-tag® II-tagged proteins for AlphaLISA no-wash assays.
AlphaLISA immunoassay kits

Glucagon (human) AlphaLISA Detection Kit

The AlphaLISA® Glucagon Detection Kit is designed for detection and quantitation of glucagon in cell culture media, sera and plasma, in a homogeneous (no-wash steps, no separation steps) assay.

TSLP (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse Thymic stromal lymphopoietin (TSLP) enables the quantitative determination of mouse TSLP in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLisa Analyte 2

Aβ 1-40 (human), lyophilized (AlphaLISA)

Human Aβ 1-40 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human Aβ 1-40 Detection Kit, but can be ordered separately.

DELFIA Eu-N1-amino Chelate and Europium (Eu) Standard, 1mg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The amino-activated chelate reacts with free carboxyl groups.
Protein

Perlecan (human), lyophilized (LANCE)

Human perlecan standard, for use in running standard curves in LANCE Ultra TR-FRET detection assays. This standard is already provided in the LANCE Ultra Human Perlecan Detection Kit, but can also be ordered separately.
PerkinElmer

LANCE Eu-W1024 Streptavidin

Europium-labeled streptavidin, for capturing biotinylated biomolecules in LANCE TR-FRET assays.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit - 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.
AlphaLISA immunoassay kits

PDGF (BB dimer) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PDGF (BB homodimer) enables the quantitative determination of human platelet-derived growth factor (PDGF) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

CYR61 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human cysteine rich angiogenic inducer 61 (CYR61) enables the quantitative determination of human Cyr61 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaScreen Acceptor Beads

AlphaScreen Protein A Acceptor beads, 25 mg

AlphaScreen Acceptor beads conjugated to Protein A.

ApoE (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoE enables the quantitative determination of Apolipopriein E (ApoE) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimum cross-reactivity with ApoA1, ApoB, and ApoCIII. Crossreactivity with other species has not tested.

Anti-methyl-Histone H3 Lysine 4 (H3K4me1-2) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 mono- or di-methylated at lysine 4 (H3K4me1-2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
AlphaLISA Cellular Epigenetics kits

AlphaLISA Acetylated-Histone H3 Lysine 27 (H3K27ac) Cellular Detection Kit

This kit is designed for the detection of acetylated Histone H3 Lysine 27 (H3K27ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

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