PerkinElmer

GPCR

OF

G蛋白偶联受体(GPCR)是结构变化多样的一大类蛋白膜受体,包括:β1和β2肾上腺素能受体、多巴胺能受体和胆碱能受体。我们可提供G蛋白偶联受体研究所需的所有先进检测分析设备。

以及多种靶标Gs-、Gq-和Gi-偶联受体的实验试剂。 我们产品还包括:

  • 荧光、发光或吸收光检测所需的可调焦GPCR细胞成像仪和液体工作站;
  • 经优化的GPCR细胞水平检测平台
  • 多样检测试剂——GPCR稳定表达细胞系、冷冻即用型GPCR细胞株及细胞膜制品,以及放射性配体等
  • 我们完备的产品组合能够满足GPCR研究的全部需求。您可借助以上仪器及试剂产品进行如下GPCR的研究应用:
  • GPCR通路激活研究:细胞内钙流、cAMP、磷酸化ERK,肌醇磷脂IP变化等
  • GPCR信号通路报告基因试验
  • 受体配体结合
  • GTP-γ-S结合活性

  • 产品与服务

  • 资源,活动及更多信息

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产品类型
  • Reagents (83)
  • Instruments (3)
  • Accessories (3)
应用
  • Drug Discovery & Development (39)
  • cAMP (8)
  • Kinase (5)
  • Protein Analysis & Detection (5)
  • Multiplexing (2)
检测点(200-50,000)
  • 50000 (10)
  • 10000 (10)
  • 500 (9)
  • 100 (2)
  • 1000 (1)
检测目标
  • Akt1/2/3 (4)
  • ERK1/2 (4)
  • cAMP (3)
  • Akt1 (2)
  • Akt1/2 (1)
  • CREB (1)
检测目标类
  • GPCR (47)
  • Phosphoprotein (11)
  • Protein (3)
检测验证
  • Binding (35)
  • Calcium Luminescence (21)
  • cAMP (5)
  • Downstream Phosphorylation (4)
  • Calcium Fluorescence (4)
自动化兼容
  • Yes (18)
浓度
  • 1.0 - 10 mCi/mL (2)
  • 50 µCi/mL (1)
  • 83.8 µCi/mL (1)
  • 87.6 µCi/mL (1)
  • 1.12 mCi/mL (1)
  • <1.0 µCi/mL (1)
检测方法
  • Alpha (14)
  • Radiometric (10)
  • Time-Resolved Fluorescence (TRF) (5)
  • Absorbance (3)
  • Fluorescence (3)
  • Luminescence (3)
  • Fluorescence Polarization (2)
  • TR-FRET (2)
  • Image-based Cytometry (1)
实验类型
  • In vitro (11)
格式
  • Microplates (14)
宿主细胞
  • CHO-K1 (28)
  • HEK293 (23)
  • 1321N1 (2)
  • A9 (1)
  • U373 (1)
  • L Cells (1)
  • UC11 (1)
  • HT29 (1)
标签位置
  • Nominally Labeled (7)
  • Specifically Labeled (3)
温度-最高
  • 40-50 (2)
  • 50-60 (2)
分子修饰
  • Phosphorylation (7)
产品品牌名称
  • Membrane Target Systems (32)
  • AequoZen (14)
  • AequoScreen (7)
  • AlphaScreen SureFire (7)
  • NEN Radiolabeled Ligands (7)
  • cAMPZen (5)
  • NEN Radiochemicals (3)
  • AlphaLISA SureFire Ultra (3)
  • EnSpire (2)
  • Alpha SureFire Ultra Multiplex (2)
  • AlphaPlex (2)
  • EnVision (2)
  • LANCE (1)
  • AlphaScreen (1)
  • LANCE Ultra (1)
  • Victor (1)
  • EnSight (1)
放射性核素
  • 125I (7)
  • 3H (2)
放射性同位素
  • I-125 (7)
  • H-3 (2)
  • C-14 (1)
受体类型
  • Adenosine (13)
  • Adrenergic (5)
  • 5-Hydroxytryptamine (Serotonin) (4)
  • Opioid (4)
  • GABA (4)
  • Vasopressin (4)
  • 5-Hydroxytryptamine (2)
  • Melanocortin (2)
  • Neurotensin (2)
  • Urotensin (1)
  • Prostanoid (1)
  • Cannabinoid (1)
  • Calcitonin (1)
  • Histamine (1)
  • Dopamine (1)
  • Glucagon (1)
  • Motilin (1)
  • Insulin (1)
  • Vasoactive Intestinal Peptide (1)
  • Bradykinin (1)
  • Galanin (1)
  • Anaphylatoxin (1)
  • Tachykinin (1)
目标种
  • Human (10)
治疗领域
  • Central Nervous System (33)
  • Inflammation (32)
  • Cardiovascular (29)
  • Metabolic (16)
  • Gastrointestinal (14)
  • Pain (13)
  • Diabetes (7)
  • Obesity (2)
  • Renal (2)
  • Angiogenesis (1)
  • CNS & Neurologic (1)

"GPCR" 1-5089 产品与服务

EnVision 2105 multimode plate reader

EnVision 多模式读板仪

EnVision 多模式读板仪在所有检测模式下都能提供最快的检测速度、超高通量及最高的灵敏度,为您的高通量筛选提供可靠的仪器及结果,以满足您日益加速的新实验需求。
VictorX_ImageA_320px

VICTOR 多模式读板仪

新一代的 VICTOR X 系列多模式读板仪为各种规模的实验室提供集灵活性、高速度和高性能于一体的解决方案。
EnSight Multimode Plate Reader

EnSight 多模式读板仪

EnSight 多模式读板仪是一台整合了多标记、无标记和显微成像技术的桌面式一体化检测平台。其整孔成像&在线数据分析速度非常快,可以在五分钟内完成384孔板整板的图像获取及分析。该仪器采用预置模块化设计,结合基于Kaleido软件的数据采集和分析流程,是一台真正的全能且高产的多模式读板仪。
Alpha Bungarotoxin

Insulin (human, recombinant), [125I]

[125I]-Insulin (h) is suitable for the use in radioimmunoassay as well as receptor binding assay. [125I]-Insulin (h) is radioiodinated with no carrier added 125I using the LPO iodination procedure and is purified by reversed phase HPLC.
Receptor-expressing cell lines

AequoZen Frozen cells, Adenosine α2A, Human Recombinant, HEK293

Our frozen, irradiated AequoZen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light. No cell culture is required prior to testing, for these frozen cells.
Alpha Bungarotoxin

GLP-1 (7-36), [125I]

Glucagon-like peptide-1 (GLP-1), labeled with 125I. GLP-1 is a peptide hormone involved in secretion of insulin, and binds and activates GLP-1 receptors, a member of the glucagon receptor family of GPCRs.
Receptor-expressing cell lines

AequoZen FroZen cells, Adrenergic α2A, Human Recombinant, CHO-K1

Our frozen, irradiated AequoZen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light. No cell culture is required prior to testing, for these frozen cells.
SureFire principle

AlphaScreen SureFire Akt 1 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
PerkinElmer

Membrane Target Systems: Neurotensin NTS1(Leu194) (human) membrane preparation, in CHO-K1 cells. 400 Units.

Cell membranes prepared from CHO-K1 cells stably-transfected with human neurotensin NTS1 (Leu194) receptor, 400 units.
Receptor-expressing cell lines

Adrenergic alpha 2A AequoScreen cell line

Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.
PerkinElmer

Membrane Target Systems: Cannabinoid CB2 (human) membrane preparation

Cell membranes prepared from cells stably-transfected with human Cannabinoid CB2 receptor, in HEK-293 cells.
SureFire principle

AlphaScreen SureFire p-Akt 1/2/3 (Thr308) research kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
PerkinElmer

LANCE cAMP Detection Kit

LANCE® TR-FRET cAMP assay kit, for Gs- and Gi-coupled GPCR functional assays.
AlphaScreen cAMP assay principle

AlphaScreen cAMP Detection Kit

AlphaScreen no-wash assay kit for quantitative detection of cAMP.
Receptor-expressing cell lines

Adenosine A3 AequoScreen® cell line

Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.
SureFire principle

Phospho-ERK (Thr202/Tyr204) Monoclonal AlphaScreen SureFire Assay kit

The AlphaScreen® SureFire® p-ERK1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK1/2 (phosphorylated on Thr202/Tyr204) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
SureFire principle

AlphaScreen SureFire Akt 1/2 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt 1/2 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt 1/2 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
PerkinElmer

Membrane Target Systems: Opioid Delta (human) membrane preparation, in HEK-293 cells. 400 Units.

Cell membranes prepared from HEK293 cells stably-transfected with human opioid delta receptor, 400 units.
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (S473) (Eu) + Total Akt1 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (Ser473) + Total Akt 1 assay kit is used to measure both the phosphorylation (Ser473) and total levels of endogenous Akt in cellular lysates. The kit measures phosphorylation on Ser473 of Akt 1/2/3, and total levels of Akt 1 only. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) activation by either recombinant or endogenous receptors, and can be applied to primary cells.
PerkinElmer

Membrane Target Systems: Vasopressin V2 (human) membrane preparation, in CHO-K1 cells. 400 Units.

Cell membranes prepared from CHO-K1 cells stably-transfected with human Vasopressin V2 receptor, 400 units.
PerkinElmer

Membrane Target Systems: Thromboxane TP (human) membrane preparation

Cell membranes prepared from cells stably-transfected with human Thromboxane TP receptor, in HEK-293 cells.
Alpha Bungarotoxin

Motilin, [125I]

Motilin, labeled with 125I. Motilin is a peptide hormone that binds motilin receptors, and stimulates contraction of gut smooth muscle.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-CREB (Ser133) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.
Diclofenac sodium- Phenylacetic acid C14

Hydroxytryptamine Binoxalate (Serotonin Binoxalate), 5-[1-14C]

5-Hydroxytryptamine Binoxalate (Serotonin Binoxalate), labeled with C-14 at the 1-position. 5-Hydroxytryptamine is a neurotransmitter and is the endogenous ligand for serotonin receptors. NEC855 replaces product NEC225, which was labeled at the 2-position.
Receptor-expressing cell lines

AequoZen frozen cells Starter Kit

AequoZen cells expressing the histamine H1 or the muscarinic M5 receptor for flash-luminescence assay.
PerkinElmer

Membrane Target Systems: U-373 cell membranes

Wild Type Subtype U-373 (human) Control Membrane Frozen suspension cell membrane. Shipped on dry ice.
Receptor-expressing cell lines

cAMPZen Frozen cells, Adenosine A2B, Human Recombinant, HEK293

Our frozen, irradiated cAMPZen cells express a variety of GPCRs for binding and functional assays. No cell culture is required prior to testing.
SureFire principle

AlphaScreen SureFire Phospho-AKT (Thr308) Kit

The AlphaScreen® SureFire® p-Akt1 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Thr308) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Receptor-expressing cell lines

Adenosine A2B AequoScreen; cell line

Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.
Adenosine 5 Triphosphate 250 µCi

(+)-Pentazocine, [Ring-1,3-3H]

(+)-Pentazocine radioligand tritiated at the 1,3 position on the ring. Pentazocine is a synthetic opioid shown to bind sigma 1 receptor.
Alpha Bungarotoxin

2'-deoxycytidine-5'-triphosphate, 5-[125I]Iodo-

Deoxycytidine triphosphate (dCTP) is a nucleoside triphosphate that is used whenever DNA is synthesized, such as in the polymerase chain reaction. 125I dCTP may be used to produce cDNA transcripts of viral or mRNA.
PerkinElmer

Membrane Target Systems: GABA B1b (human) membrane preparation, in CHO-K1 cells. 400 Units.

Cell membranes prepared from CHO-K1 cells stably-transfected with human GABA B1b receptor, 400 units.
PerkinElmer

Membrane Target Systems: Serotonin 5HT6 (rat) membrane preparation

Cell membranes prepared from cells stably-transfected with rat Serotonin 5-HT6 receptor, in HEK-293 cells.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-AKT1/2/3 (Thr308) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-ERK 1/2 (Eu) + Total ERK 545 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-ERK 1/2 + Total ERK assay kit is used to measure both the phosphorylation (Thr202/Tyr204) and total levels of endogenous “extracellular signal-regulated kinase 1 and 2” (ERK 1/2) in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure ERK 1/2 activation by either recombinant or endogenous receptors, and can be applied to primary cells.
PerkinElmer

LANCE Ultra cAMP Detection Kit

The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).
PerkinElmer

Membrane Target Systems: GABA B1b in HEK (human) membrane preparation, in HEK-293 cells. 400 Units.

Cell membranes prepared from HEK293 cells stably transfected with human GABA B1b receptor, 400 units.
Alpha Bungarotoxin

CGRP (human), [125I]

Calcitonin gene-related peptide (CGRP), labeled with 125I. CGRP binds calcitonin receptors.
Receptor-expressing cell lines

cAMPZen Frozen cells, Adenosine A1, Human Recombinant, CHO-K1

Our frozen, irradiated cAMPZen cells express a variety of GPCRs for binding and functional assays. No cell culture is required prior to testing.
Alpha Bungarotoxin

N-succinimidyl-3-(4-hydroxy-3-[125I]iodophenyl)propionate, Bolton-Hunter Reagent

Bolton-Hunter reagent, monoiodinated. This product is used to iodinate proteins that do not contain tyrosine residues. Bolton-Hunter reagent is the N-hydroxysuccinimide ester of iodinated p-hydroxyphenylpropionic acid. The active ester acylates terminal amino groups with the iodinated p-hydxyphenylpropionic residue, effectively introducing radioactive iodine into proteins and peptides. A non-oxidative technique, it is less harsh to proteins than alternative labeling methods. An anhydrous benzene A charcoal trap is provided with each vial.
Receptor-expressing cell lines

cAMPZen Frozen cells, Adenosine A3, Human Recombinant, CHO-K1

Our frozen, irradiated cAMPZen cells express a variety of GPCRs for binding and functional assays. No cell culture is required prior to testing.
PerkinElmer

Membrane Target Systems: Neurotensin NTS1 (human) membrane preparation

Cell membranes prepared from cells stably-transfected with human Neurotensin NTS1 receptor, in HEK-293 cells.
Receptor-expressing cell lines

AequoZen FroZen cells, Parental, CHO-K1 (+Gα16, medium level)

AequoScreen Parental cell stably expressing the mitochondrially targeted apoaequorin gene. GPCRs of your choice can be transiently or stably transfected into these cell lines, for luminescence calcium assays.
PerkinElmer

Membrane Target Systems: Vasopressin V1b (human) membrane preparation, in CHO-K1 cells. 400 Units.

Cell membranes prepared from CHO-K1 cells stably-transfected with human Vasopressin V1b receptor, 400 units.
PerkinElmer

Membrane Target Systems: Urotensin UR2 (GPR14) (mouse) membrane preparation

Cell membranes prepared from cells stably-transfected with human Urotensin UR2 (GPR14) receptor, in CHO-K1 cells.
Receptor-expressing cell lines

AequoZen FroZen cells, Adenosine A1, Human Recombinant, CHO-K1

Our frozen, irradiated AequoZen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light. No cell culture is required prior to testing, for these frozen cells.
PerkinElmer

Membrane Target Systems: GABA B1a (human) membrane preparation, in CHO-K1 cells. 400 Units.

Cell membranes prepared from CHO-K1 cells stably-transfected with human GABA B1a receptor, 400 units.
Receptor-expressing cell lines

AequoZen FroZen cells, Parental, 1321N1

AequoScreen Parental cell stably expressing the mitochondrially targeted apoaequorin gene. GPCRs of your choice can be transiently or stably transfected into these cell lines, for luminescence calcium assays.
alphascreen surefire total

Total ERK (monoclonal) AlphaScreen SureFire Assay Kit

The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

"GPCR" 1-5089 产品与服务