PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

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How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. http://www.perkinelmer.com/ASK/LANCE.

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"LANCE TR-FRET" 1-50367 产品与服务

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LANCE Ultra ULight™-JAK-1 (Tyr1023) Peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from amino acids 1015-1027 of human Janus kinase 1 (JAK-1). The ULight-JAK-1 peptide can be phosphorylated at Tyr1023 by JAK3 and several other tyrosine kinases. Peptide sequence: CAGAGAIETDKEYYTVKD

ApoA1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ApoA1 Detection Kit is designed for detection and quantitation of human Apolipoprotein A1 (ApoA1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

LANCE® Eu-W1024 Anti-phosphotyrosine (P-Tyr-100)

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in LANCE® TR-FRET kinase assays.
AlphaLisa Analyte 2

IL-31 (human), lyophilized (LANCE)

Human IL-31 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IL-31 TR-FRET Detection Kit, but can also be ordered separately.
PerkinElmer

LANCE® Eu-W1024 Anti-phosphotyrosine (PT66)

Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.
LANCE Detection Kit

IL-1β (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-1β Detection Kit is designed for detection and quantitation of human interleuukin-1β in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE Detection Kit

EGF (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human EGF Detection Kit is designed for detection and quantitation of human epidermal growth factor in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Chorionic gonadotropin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Chorionic Gonadotropin (hCG) Detection Kit is designed for detection and quantitation of hCG in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE Detection Kit

TSH (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human TSH Detection Kit is designed for detection and quantitation of human thyroid stimulating hormone in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

LANCE Ultra ULight-TK peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing 28 amino acid peptide with 8 Tyrosine residues in different amino acid context that broadly provides for the recognition specificity of greater than 85% of 83 tested cytoplasmic and receptor tyrosine kinases.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total eIF2α LANCE Ultra TR-FRET Detection Kit

LANCE Ultra Total eIF2α kits are designed for the detection of total eIF2α (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-eIF2α detection, and is compatible with both adherent and suspension cells.

ULight anti-FITC

ULight™ labeled anti-FITC antibody for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
PerkinElmer

LANCE Ultra ULight™-PLK (Ser137) peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRRSLLE
Protein

ANGPTL4 (human), lyophilized (LANCE)

Human angiopoietin-like 4 (ANGPTL4) standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human ANGPTL4 Detection Kit, but can also be ordered separately.
Protein

p53 (human), lyophilized (LANCE)

Human p53 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra p53 Detection Kit, but can also be ordered separately.

LANCE Ultra Europium-anti-methyl-Histone H3 Lysine 4 (H3K4me1-2)

Europium-labeled monoclonal antibody recognizing human H3 mono- and di-methylated at lysine 4 (H3K4me1-2). For use in LANCE® Ultra epigenetic writer and eraser assays.
PerkinElmer

LANCE Eu-W1024 anti-FLAG

Eu-W1024 labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
LANCE_Fc_receptor

FCGR3A/CD16a (176Val/V158) LANCE Ultra TR-FRET Binding Kit

This kit is designed for the quantitative determination of Human FCGR3A/CD16a (176Val/V158) and human Fc fragment binding using a homogeneous (no wash) LANCE Ultra TR-FRET binding assay.
LANCE Detection Kit

CCL2/MCP-1α (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse CCL2/MCP-1α Detection Kit is designed for detection and quantitation of mouse Monocyte Chemoattractant Protein 1α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-eIF2A (Ser51) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-eIF2A (Ser51) kits are designed for the detection of eIF2A (phosphorylated at S51) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
PerkinElmer

LANCE Eu-W1024 Streptavidin

Europium-labeled streptavidin, for capturing biotinylated biomolecules in LANCE TR-FRET assays.
AlphaLisa Analyte 2

IgG (bovine), lyophilized (LANCE)

Bovine IgG standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Bovine IgG TR-FRET Detection Kit, but can also be ordered separately.
LANCE Detection Kit

Apo-CIII (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Apo-CIII Detection Kit is designed for detection and quantitation of ApoC3 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

LANCE® Eu-W1024 phospho-(Ser) 14-3-3 binding motif antibody, 10 µg

Europium-labeled anti-phospho-(Ser) 14-3-3 motif antibody, for use in LANCE® TR-FRET kinase assays.

ULight-anti-V5 (LANCE TR-FRET)

Anti-V5 tag antibody labeled with ULight™ dye. This ULight-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® Europium-labeled reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total STAT3 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total STAT3 kits are designed for the detection of total STAT3 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-STAT3, and is compatible with both adherent and suspension cells.
LANCE Eu-W8044 Streptavidin

LANCE Eu-W8044 Streptavidin

Europium-labeled streptavidin, for capturing biotinylated biomolecules in LANCE TR-FRET assays. The W8044 chelate provides more stability than the W1024 chelate.

LANCE Ultra ULight™-Streptavidin

Streptavidin labeled with ULight™ dye.

Leptin (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse Leptin Detection Kit is designed for detection and quantitation of mouse leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AKT 1 (Thr308) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-AKT1 (Thr308) kits are designed for the detection of AKT1 (phosphorylated at T308) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
LANCE Detection Kit

ApoE (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ApoE Detection Kit is designed for detection and quantitation of human Apolipoprotein E (ApoE) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

EGFR (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

ULight anti-mouse IgG

ULight™ labeled anti-mouse IgG antibody for LANCE® TR-FRET assays. The anti-mouse IgG antibody is a purified goat polyclonal antibody.
LANCE Detection Kit

IL-6 (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse IL-6 Detection Kit is designed for detection and quantitation of murine IL-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

IL-1β (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse IL-1β Detection Kit is designed for detection and quantitation of mouse interleukin 1β in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AKT 1/2/3 (Ser473) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total ERK 1/2 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra Total ERK 1/2 kits are designed for the detection of total (phosphorylated and non-phosphorylated) ERK1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-ERK detection, and is compatible with both adherent and suspension cells.
PerkinElmer

Biotinylated human FCGR3A (176Val) (LANCE)

Biotinylated human FCGR3A/CD16a(176Val/V158) for use in LANCE Ultra TR-FRET Fc-receptor binding assays.

LANCE Ultra Europium anti-phospho-Glycogen Synthase (Ser641)

Europium-labeled rabbit polyclonal antibody recognizing human Muscle Glycogen Synthase phosphorylated at Ser641. Antibody labeled with the LANCE® Eu-W1024-ITC chelate. Sequence motif recognized: PApSVPPSPSL

PD-L1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human PD-L1 Detection Kit is designed for detection and quantitation of human PD-L1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
PerkinElmer

LANCE Ultra NFκB Positive Control Lysate

Control lysates for LANCE Ultra TR-FRET cellular phospho-NFκB (Ser536) and Total NFκB detection assays.
LANCE Detection Kit

IL-17A (mouse/rat) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse/Rat IL-17A Detection Kit is designed for detection and quantitation of mouse or rat interleukin 17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

CCL3/MIP1α (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human CCL3/MIP1α Detection Kit is designed for detection and quantitation of human CCL3 (MIP1α) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

CHO-P LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra CHO-P Detection Kit is designed for detection and quantitation of CHO proteins in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

CXCL9/MIG (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human CXCL9/MIG Detection Kit is designed for detection and quantitation of human CXCL9/MIG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE Detection Kit

Nidogen 1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Nidogen 1 Detection Kit is designed for detection and quantitation of Nidogen 1 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

TNFα (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse TNFα Detection Kit is designed for detection and quantitation of mouse tumor necrosis factor α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence: CSERRPHFPQFSYSASGTAR

LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium chelate. This antibody detects phospho-Ser in a synthetic peptide (RFARKGSLRQKNV) only after the peptide is phosphorylated by PKC or PKA kinases. The synthetic peptide is derived from amino acids 19-31 of human PKCα in which Ala25 is mutated to Ser. Sequence motif recognized: RFARKG-pS-LRQKNV

LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRQSLIE

"LANCE TR-FRET" 1-50367 产品与服务

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