PerkinElmer
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  • 应用基因组学

Molecular Cytogenetics

Fast, precise, and cost-effective targeted molecular karyotyping

Molecular cytogenetics plays an important role in the characterization of chromosome anomalies and is a critical component of current genetic studies including research into both constitutional disorders and cancer.

PerkinElmer is bringing the newest molecular techniques to cytogenetic laboratories. Our innovative methodologies support:

  • Increased detection rates
  • Faster results at lower costs
  • Streamlined processes
  • Support from sample-to-result

Oligonucleotide-based microarrays and bead-based multiplex BACs-on-Beads® products have been specifically designed, developed and validated by cytogeneticists for cytogeneticists performing molecular karyotyping.

For research use only. Not for use in diagnostic procedures.

Applications

细胞遗传学试剂 - 研究

Fast, precise, and cost-effective targeted molecular karyotyping

BACs are Bacterial Artificial Chromosomes, large cloned sequences of human DNA typically 170,000 bases long. BAC probes have long been used for FISH. The BACs-on-Beads® technology uses probes generated from selected BACs immobilized onto Luminex® xMAP® encoded beads. The resulting bead sets are used to assay chromosomal gains and losses from minute sample amounts with high throughput. BACs-on-Beads analysis comprises hybridization of DNA sample sequences to BAC-derived probes representing the identified target regions. Samples are enzymatically labeled with biotin, and after hybridization in a 96-well plate a fluorescent streptavidin-phycoerythrin reporter is bound to the biotin label. Male and female reference DNA is also hybridized and the fluorescence ratio between each sample and reference DNA are compared to provide a clear display of any copy number changes in the targeted regions. Sample labeling is done enzymatically, which provides significant amplification of samples down to 100-200 ng.

For research use only. Not for use in diagnostic procedures.

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Cytogenetic Microarrays

Cytogenetic microarrays offer a simple, reliable method for assessing chromosomal aberrations at a high resolution. PerkinElmer’s CGX microarrays detect chromosome aberrations and provide accurate, and comprehensive, genome-wide analysis in a rapid and cost effective manner.

For research use only. Not for use in diagnostic procedures. These products are not available in the USA.

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