PerkinElmer
  • 研究领域
  • 应用基因组学

Protein Characterization

Sample preparation and characterization of proteins for quality by design (QbD) studies are important parts of process development; however, these studies, including design of experiments(DoE), can be time consuming and sample analysis can easily exceed the capacity of most labs.

Protein characterization involves both upstream protein purification optimization, as well as the downstream characterization of its purity, structure and function. Miniaturization of small-scale protein purification provides greater process optimization with larger QbD and DOE studies allowing for parallel processing of samples for increased statistical significance and greater breadth of variables within experimental shorter time frames.

PerkinElmer is committed to accelerating this process to help life science researchers make critical decisions earlier in the protein development workflow. Researchers can now screen for optimal protein characteristics earlier in the process, integrating Quality by Design initiatives into their protein therapeutic development workflow.

For research use only. Not for use in diagnostic procedures.

Solutions

Microfluidic Protein Characterization Analysis

Microfluidic Electrophoresis Analysis

Proteins differ from each other in their size, molecular structure and physiochemical properties. These differences allow for protein analysis and characterization by separation and identification. The LabChip® GXII Touch protein characterization system offers rapid peak quantification and quality control throughout the protein purification workflow.

The platform supports multiple assays for characterizing proteins in reduced and non-reduced samples including:

  • Analyze critical quality attributes in as few as 42 seconds:
  • Titer (concentration and sizing)
  • Purity assessment
  • Aggregation (covalently linked)
  • Stability (degradation / fragmentation analysis)
  • N-Glycan profiling
  • mAb Charge variant / charge heterogeneity
  • mAb impurity analysis (detect minor impurities)

Analysis can be performed in as few as forty seconds per sample that deliver comparable data to traditional capillary electrophoresis with as much as a 70X increase in throughput. Choose 96-well or up to 384-well platforms depending upon throughput needs. With an easy to use touch screen interface, even occasional users get up and running samples quickly.

For research use only. Not for use in diagnostic procedures.

Learn more

Automated Small Scale Protein Purification

Small scale protein purification presents opportunities for accelerated process development of biotherapeutic molecules. Miniaturization of purification conditions reduces time and allows for parallel processing of samples offering increased statistical significance and greater breadth of variables. Critical to the utility of this approach is the ability of the miniaturized platform to be predictive of larger scale purification schemes.

For research use only. Not for use in diagnostic procedures.

Learn more

微孔板读板仪

PerkinElmer’s portfolio of microplate readers are all equipped with multiple detection modes to meet the diverse assay requirements of today’s laboratories. No matter which one of our plate readers you choose, you can expect excellent performance and reliability. The variety of models and accessories, such as plate stackers, means there’s a PerkinElmer plate reader to match both your throughput needs and your budget.

You can also benefit from our greater than 30 years’ experience in assay and instrument development, and our vast array of complementary products (assays, microplates, reagents, and informatics), and services (tech support and instrument maintenance and servicing) for a complete solution for your research.

For research use only. Not for use in diagnostic procedures.

Learn more

高内涵筛选仪器和显微镜

Get the sensitivity and throughput you need to image more samples and analyze more parameters with high content screening. Our high content screening instruments help you answer the most complex biological questions. With powerful yet simple imaging and analysis capabilities for a wide range of applications -- from basic research to assay development and screening -- these powerful high content screening microscope systems produce the highest possible image quality to take your research further, in less time than ever before, especially when combined with PerkinElmer's robotic systems and advanced data analytics.

For research use only. Not for use in diagnostic procedures.

Learn more