PerkinElmer

蛋白质检测

OF

Detect and quantitate proteins across a variety of sample types. We offer a broad range of products including traditional western blotting reagents and consumables, radioimmunoassay (RIA), ELISA, and ELISA alternative assays, tissue imaging systems and immunohistochemistry reagents, and innovative microfluidic technology. Choose from a variety of platforms and techniques to detect proteins with optimal performance, ease-of-use, and highest throughput depending on your needs.

  • Sensitive and reliable detection with western blotting, the gold standard of protein detection
  • Analyze proteins quickly and simply using our fast, simple, pre-validated kits and multimode plate readers for highly-sensitive protein quantitation in singleplex or multiplex, in a variety of sample types
  • Amplified signal detection of proteins in tissue samples with our singleplexing and multiplexing immunohistochemistry (IHC) reagents
  • Sample acquisition times less than 40 seconds (96 samples in less than an hour) in our leading microfluidics platform virtually eliminate throughput bottlenecks and improve efficiency

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"蛋白质检测" 1-501104 产品与服务

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16065 PE DropletQuant

DropletQuant, UV-VIS Spectrophotometer

The DropletQuant complements PerkinElmer's existing LabChip microfluidics instrument portfolio, which provide electrophoretic separation and analysis of nucleic acids, proteins and glycan profiling.

Key Applications:

  • DNA, RNA and protein microvolume quantitation
  • Impurity analysis; removal of buffer and contaminants from calculations of biomolecules of interest using cDrop™ software
  • Purity calculations
  • Measurement of dye labeling efficiency of microarray samples
  • Color measurements (Bradford Assay)
  • General UV/VIS measurements
AlphaLISA SureFire Ultra kits

Total Cofilin AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra Total Cofilin assay is a sandwich immunoassay for quantitative detection of cofilin (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
LANCE Detection Kit

MANF (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human MANF Detection Kit is designed for detection and quantitation of human MANF in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SYK (Tyr525/526) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SYK (Tyr525/526) in a multiplexed assay.
PerkinElmer

LANCE Eu-W1024 Anti-HA

Europium-labeled anti-HA antibody, for capturing HA-tagged proteins and peptides in LANCE® TR-FRET assays.
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr70) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr70) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr70) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Protein

IgA (human), lyophilized (LANCE)

Human IgA standard, for use in running standard curves in LANCE® Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IgA Detection Kit, but can also be ordered separately.
AlphaLISA Cellular Epigenetics kits

AlphaLISA Acetylated-Histone H3 Lysine 27 (H3K27ac) Cellular Detection Kit

This kit is designed for the detection of acetylated Histone H3 Lysine 27 (H3K27ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

CCL2 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human CCL2/MCP-1 Detection Kit is designed for detection and quantitation of human CCL2/MCP-1 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
AlphaLisa Analyte 2

ApoB (human), lyophilized (AlphaLISA)

Human ApoB standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human ApoB Detection Kit, but can also be ordered separately.

LANCE® Eu-W1024 Anti-Rabbit IgG

Europium-labeled anti-rabbit IgG, for capturing antibodies in LANCE® TR-FRET assays.

PIP Collagen (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra PIP Collagen Detection Kit is designed for detection and quantitation of human PIP collagen in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Western Lightning ECL Pro chemiluminescent substrates for western blotting

Western Lightning ECL Pro

Western blotting is the most widely used technique for detecting specific proteins. PerkinElmer brings you a wide range of western blotting solutions. Now, you can have the highest level of sensitivity, specificity and confidence in your results. By offering a unique combination of specific immunodetection and size-based separation, western blotting gives you reliable, convenient, high-quality data.
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (S473) (Eu) + Total Akt1 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (Ser473) + Total Akt 1 assay kit is used to measure both the phosphorylation (Ser473) and total levels of endogenous Akt in cellular lysates. The kit measures phosphorylation on Ser473 of Akt 1/2/3, and total levels of Akt 1 only. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) activation by either recombinant or endogenous receptors, and can be applied to primary cells.

PCSK9 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human PCSK9 Detection Kit is designed for detection and quantitation of human Proprotein Convertase Subtilisin/Kexin Type 9 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

ULight anti-human IgG

ULight™ labeled anti-human IgG antibody for LANCE® TR-FRET assays. The anti-human IgG antibody is a purified goat polyclonal antibody, and reacts with whole molecule human IgG.
AlphaLISA immunoassay kits

ApoE (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoE enables the quantitative determination of Apolipopriein E (ApoE) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimum cross-reactivity with ApoA1, ApoB, and ApoCIII. Crossreactivity with other species has not tested.
AlphaLisa Analyte 2

Hepatitis B Virus s Antigen (HBsAg), lyophilized (AlphaLISA)

Hepatitis B Virus Surface antigen (HBsAg) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA HBsAg Detection Kit, but can also be ordered separately.
AlphaLISA immunoassay kits

Myeloperoxidase (human) AlphaLISA Detection Kit

The AlphaLISA® Human Myeloperoxidase (MPO) Detection Kit is designed for detection and quantitation of human MPO in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

GAD67 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for GAD67 enables the quantitative determination of human glutamate decarboxylase 67 (hGAD67) in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows less than 5% cross reactivity with mouse GAD67. Cross-reactivity with other species has not tested.
PerkinElmer

LANCE Eu-W1024 Anti-c-Myc

Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.
AlphaLISA immunoassay kits

Nidogen-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human Nidogen-1 enables the detection and quantitation of Human Nidogen-1 (NID-1; also known as Entactin) in human serum, plasma, and, cell lysyate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total SYK Assay Kit

The AlphaLISA® SureFire® Ultra Total SYK assay is a sandwich immunoassay for quantitative detection of SYK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AKT 1/2/3 (Thr308) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-AKT 1/2/3 (Thr308) kits are designed for the detection of phosphorylated AKT1, AKT2, and AKT3 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
AlphaScreen Acceptor Beads

AlphaScreen Unconjugated Acceptor Beads

Unconjugated AlphaScreen® Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaScreen Acceptor beads for use in Alpha no-wash assays.
LANCE Detection Kit

MMP-8 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human MMP-8 Detection Kit is designed for detection and quantitation of human matrix metalloproteinase 8 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
alphaimmunoassay1

IL-6 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin-6 (IL6) Detection Kit is designed for detection and quantitation of human IL-6 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

Anti-Mouse IgE (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgE. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
AlphaLisa Analyte 2

Somatostatin (human), lyophilized (AlphaLISA)

Somatostatin standard, solid, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Somatostatin kit, but can be ordered separately.

Anti-Mouse IgG3 (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG3. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
AlphaLISA immunoassay kits

IgG (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for detection and quantitation of human IgG in buffer or cell culture supernatant allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development. The antibodies in the kit target the Fcγ region.
SureFire principle

AlphaScreen SureFire Phospho-GSK 3a (Ser21) Kit

The AlphaScreen® SureFire® p-GSK 3α (Ser21) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3α (phosphorylated on Ser21) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH Assay Kit

The AlphaLISA® SureFire® Ultra™ Total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for cell number.

AlphaScreen FITC (Fluorescein) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FITC conjugated AlphaScreen Acceptor beads. 

VEGF (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human VEGF Detection Kit is designed for detection and quantitation of human VEGF in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Anti-methyl-Histone H3 Lysine 4 (H3K4me1-2) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 mono- or di-methylated at lysine 4 (H3K4me1-2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IP10 Detection Kit

The AlphaPlex Samarium (Sm) Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

FcγR2A/CD32a (167R) AlphaLISA Binding Kit

This kit is designed for the detection of binding between FCGR2A (CD32a) (167R) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics by using competitive binding.

AlphaLISA Biotin-anti-Histone H4 Antibody

AlphaLISA® biotinylated monoclonal antibody recognizing the amino-terminal end of human histone H4. Broad species cross-reactivity is expected based on sequence similarity. This product is designed to be used with AlphaLISA® Epigenetics Acceptor beads to detect modified full-length histone H4 in homogeneous AlphaLISA assays.

LANCE Ultra ULight™-anti-6xHis

Mouse monoclonal anti-polyHistidine tag antibody labeled with ULight™ dye.

IL-6 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interleukin-6 (IL-6) Detection Kit is designed for detection and quantitation of mouse IL-6 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit is a superior alternative to a traditional mouse IL-6 ELISA kit due to its simple and fast protocol, wide dynamic range, and high sensitivity.
AlphaLISA immunoassay kits

FCGR1/CD64 AlphaLISA Binding Kit

This kit is designed for the detection of binding between FCGR1 (CD64) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
AlphaLISA immunoassay kits

HLA-E (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human HLA-E enables the quantitative determination of human MHC class I antigen E (HLA-E) in buffered solution, cell culture supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human/Rat IL22 Detection kit

The AlphaPlex 545 (Terbium) Human / Rat IL22 Detection Kit is designed for the quantitative determination of human or rat IL22 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
AlphaPlex Terbium immunoassay kits

AlphaPlex™ 545 IL-6 Detection Kit 

The AlphaPlex™ 545 (Terbium) IL-6 Detection Kit is designed for the quantitative determination of human IL6 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-eIF4E (Ser209) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-eIF4E (Ser209) in a multiplexed assay.
LANCE Detection Kit

EGFR (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

Corticosterone (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human corticosterone enables the quantitative determination of corticosterone in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLisa Analyte 2

DNA (liquid), AlphaLISA

DNA standard for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Host Cell Residual DNA kit, but can be ordered separately.

AlphaScreen C-MYC Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-c-myc-conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.

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