PerkinElmer

TR-FRET标记试剂

OF

Develop your own TR-FRET custom assays and label your biomolecule with a Europium lanthanide chelate. LANCE® Europium chelates use the following reactive groups for labeling chemistry:

  • Isothiocyanate (ITC)-activated chelates react primarily with free amine groups, including the N-terminus of a protein or peptide as well as lysine residues on antibodies, proteins, and peptides.
  • Iodoacetamide (IA)-activated chelates react with free sulfhydryl groups (including cysteines) on peptides and proteins to form a stable, covalent thio-ether bonds.
  • Diochlorotriazine (DTA)-activated chelates react with free amino, sulfhydryl, and hydroxyl groups and are suitable for labeling proteins, peptides, nucleic acids, and small molecules containing these functional groups.

LANCE Eu-W1024 chelates are preferred in most High Throughput Screening (HTS) applications due to their low fluorescent background and high S:B in TR-FRET assays. For assays that require high concentrations of EDTA or other chelators, a high temperature, or a pH below 7, the Eu-W8044 chelate offers greater stability.

  • 产品与服务

  • 资源,活动及更多信息

缩小结果
荧光基团
  • Eu-W1024-ITC (2)
  • Eu-W1284-Iodoacetamido (2)
  • Eu-W8044-DTA (1)

"TR-FRET标记试剂" 1-55 产品与服务

LANCE Eu-W1024-ITC Chelate and Eu Standard, 100µg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.

LANCE Eu-W1284-Iodoacetamido Chelate and Eu Standard, 1 mg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The iodoacetamido-activated chelate reacts primarily with free sulfhydryl groups, such as cysteines.

LANCE Eu-W8044-DTA Chelate and Eu Standard, 1 mg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The DTA-activated chelate reacts with free amino groups, sulfhydryl groups, and hydroxyl groups. Because of its high reactivity, we usually do not recommend DTA as it may “inactivate” the reagent you are labeling.

LANCE Eu-W1024-ITC Chelate and Eu Standard, 1 mg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.

LANCE Eu-W1284-Iodoacetamido Chelate and Eu Standard, 100µg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The iodoacetamido-activated chelate reacts primarily with free sulfhydryl groups, such as cysteines.

"TR-FRET标记试剂" 1-55 产品与服务