Apoptosis | Cellular Imaging & Analysis | Lab Products & Services | PerkinElmer
珀金埃尔默使用cookies来确保我们为您提供在我们网站上的最佳体验。 这可能包括来自第三方网站的cookies。 如果您不改变您的设置点击继续,我们会认为您同意接收本网站的cookies。 您可以随时更改您的Cookie设置。 要了解更多信息,请查看我们的Cookie政策,其中包含有关如何管理Cookie的信息。


Apoptosis assays are a widely used application in High Content Analysis because of their central role in cancer research and immunology. There are two main pathways for the induction of apoptosis: the death receptor-mediated pathway and the mitochondrial pathway.

Induction of either pathway will result in the activation of caspases, a class of intracellular cysteine proteases which are considered to be the central components of the apoptotic response. 

Imaging based analysis of caspase activation, mitochondrial function and nuclear fragmentation are typical high content apoptosis assays. 

Apoptosis assay
The figure above shows a schematic representation of two commonly analyzed apoptotic features in High Content Screening: nuclear fragmentation and caspase-3 activation upon apoptotic stimulus. The cells have been stained with a nuclear marker (red) and an antibody for caspase-3 (green) in various stages after treatment with an apoptosis inducing compound. From left to right: increasing dose of compound.

For Research Use Only. Not for Use in Diagnostic Procedures.