HCS plays a significant role in infectious disease research as it enables high throughput functional and phenotypic assays that can be adapted to a wide range of pathogens including virus, bacteria and eukaryotic parasites. Applications include genetic siRNA interference screens for identifying host factors involved in host–pathogen interactions, but also screens for lead discovery in drug discovery. High-content analysis enables, for example, intracellular tracking of viral particles to profile the antiviral mechanisms of each compound and sensitive measurements of bacterial infection rates. Adaptation to high-throughput screening in bacteriology and parasitology has already led to the discovery of new types of host-specific inhibitors that differ from those inhibitors that act directly on microbes.

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Unravelling the Zika Virus

Publications using PerkinElmer High-Content Screening Products

• Chang SY, Park JH, Kim YH, Kang JS and Min J-Y. A natural component from Euphorbia humifusa Willd displays novel, broad-spectrum anti-influenza activity by blocking nuclear export of viral ribonucleoprotein. Biochemical and biophysical research communications. 2016; 471(2):282-9.
• Cortjens B, de Boer OJ, de Jong R, Antonis AF, Sabogal Piñeros YS, Lutter R, van Woensel J and Bem RA. Neutrophil Extracellular Traps Cause Airway Obstruction During Respiratory Syncytial Virus Disease. The Journal of Pathology. 2016; 238(3):401-411.
• Ekins S, Freundlich JS, Clark AM, Anantpadma M, Davey RA and Madrid P. Machine learning models identify molecules active against the Ebola virus in vitro.F1000 Research. 2016; 4:1091.
• Kim H-Y, Kong S, Oh S, Yang J, Jo E, Ko Y, Kim S-H, Hwang JY, Song R and Windisch MP. Benzothiazepinecarboxamides: Novel hepatitis C virus inhibitors that interfere with viral entry and the generation of infectious virions. Antiviral Research. 2016; 129:39-46.
• McMullan LK, Flint M, Dyall J, Albariño C, Olinger GG, Foster S, Sethna P, Hensley LE, Nichol ST and Lanier ER. The lipid moiety of brincidofovir is required for in vitro antiviral activity against Ebola virus. Antiviral Research. 2016; 125:71-78.
• Park JH, Park EB, Lee JY and Min J-Y. Identification of novel membrane-associated prostaglandin E synthase-1 (mPGES-1) inhibitors with anti-influenza activities in vitro. Biochemical and Biophysical Research Communications. 2016; 469(4):848-855.
• Phillips SL, Soderblom EJ, Bradrick SS and Garcia-Blanco MA. Identification of Proteins Bound to Dengue Viral RNA In Vivo Reveals New Host Proteins Important for Virus Replication. mBio. 2016; 7(1):e01865-15.
• Radoshitzky SR, Pegoraro G, Chī X, Dǒng L, Chiang C-Y, Jozwick L, Clester JC, Cooper CL, Courier D and Langan DP. siRNA Screen Identifies Trafficking Host Factors that Modulate Alphavirus Infection. PLoS Pathogens. 2016; 12:e1005466.
• Tegazzini D, Díaz R, Aguilar F, Peña I, Presa JL, Yardley V, Martin JJ, Coteron JM, Croft SL and Cantizani J. A replicative in vitro assay for drug discovery against Leishmania donovani.Antimicrobial Agents and Chemotherapy. 2016; 60(6):3524-3532.
• Alonso-Padilla J, Cotillo I, Presa JL, Cantizani J, Peña I, Bardera AI, Martín JJ and Rodriguez A. Automated High-Content Assay for Compounds Selectively Toxic to Trypanosoma cruzi in a Myoblastic Cell Line. PLoS Neglected Tropical Diseases. 2015; 9(1):e0003493.
• Blyth GA, Chan W-F, Webster RG and Magor KE. Duck IFITM3 mediates restriction of influenza viruses. Journal of Virology. 2015: 90(1):103-116.
• Caì Y, Postnikova EN, Bernbaum JG, Yú S, Mazur S, Deiuliis NM, Radoshitzky SR, Lackemeyer MG, McCluskey A and Robinson PJ. Simian hemorrhagic fever virus cell entry is dependent on CD163 and uses a clathrin-mediated endocytosis-like pathway. Journal of Virology. 2015;89(1):844-856.
• Chen J, Jiang L, Lan K and Chen X. Celecoxib Inhibits the Lytic Activation of Kaposi’s Sarcoma-Associated Herpesvirus through Down-Regulation of RTA Expression by Inhibiting the Activation of p38 MAPK. Viruses. 2015; 7(5):2268-2287.
• Delorme V, Song O-R, Baulard A and Brodin P. Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells Using Phenotypic Assays. Methods in Molecular Biology. 2015; 1285:387-411.
• Duez J, Holleran JP, Ndour PA, Loganathan S, Amireault P, Français O, El Nemer W, Le Pioufle B, Amado IF and Garcia S. Splenic retention of Plasmodium falciparum gametocytes to block the transmission of malaria. Antimicrobial Agents and Chemotherapy. 2015; 59(7):4206-4214.
• Herbert AS, Davidson C, Kuehne AI, Bakken R, Braigen SZ, Gunn KE, Whelan SP, Brummelkamp TR, Twenhafel NA and Chandran K. Niemann-Pick C1 Is Essential for Ebolavirus Replication and Pathogenesis In Vivo. mBio. 2015; 6(3):e00565-15.
• Holtsberg FW, Shulenin S, Vu H, Howell KA, Patel SJ, Gunn B, Karim M, Lai JR, Frei JC and Nyakatura EK. Pan-ebolavirus and pan-filovirus mouse monoclonal antibodies: protection against Ebola and Sudan viruses. Journal of Virology. 2015; 90(1):266-78.
• Kang H, Kim C, Kim D-e, Song J-H, Choi M, Choi K, Kang M, Lee K, Kim HS and Shin JS. Synergistic antiviral activity of gemcitabine and ribavirin against enteroviruses. Antiviral Research. 2015, 124:1-10.
• Khare S, Liu X, Stinson M, Rivera I, Groessl T, Tuntland T, Yeh V, Wen B, Molteni V and Glynne R. Anti-Trypanosomal Treatment with Benznidazole is Superior to Posaconazole Regimens in Mouse Models of Chagas Disease. Antimicrobial agents and chemotherapy. 2015; 59(10):6385-6394.
• Khare S, Roach SL, Barnes SW, Hoepfner D, Walker JR, Chatterjee AK, Neitz RJ, Arkin MR, McNamara CW and Ballard J. Utilizing Chemical Genomics to Identify Cytochrome b as a Novel Drug Target for Chagas Disease. PLoS Pathogen. 2015; 11(7):e1005058.