PerkinElmer
check quantity

α-tubulin (human) AlphaPlex Terbium Detection Kit, 100 assay points

The AlphaPlex™ Terbium (Tb) Human α-Tubulin Detection Kit is designed for the quantitative determination of human α-tubulin in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay. This assay can be used as a cell normalization or protein level normalization assay, in conjunction with AlphaLISA® biomarker detection kits.

项目已成功添加到购物车

For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AP383TB-HV
100 Assay Points
更多
AP383TB-C
500 Assay Points
更多
AP383TB-F
5,000 Assay Points
更多
立即购买

请输入有效的数量

请登录后再添加至收藏夹

购物车为空

詳情 信息

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Alpha-tubulin is a 451 amino acid protein that is one of the components of the microtubule cytoskeleton of eukaryotic cells. Alpha and beta tubulin form the skeleton itself, while gamma tubulin is involved in its organization. Alpha-tubulin is ubiquitous in every cell and tissue of the human body, and is highly preserved at the eukaryotic level (human 95% with mouse and 74% with yeast). As such, detection of alpha tubulin in cell extracts is a good tool for normalization of the amount of cell proteins per well in a biochemical assay. The alpha-tubulin AlphaLISA detection kit allows for the detection of alpha-tubulin in tissues homogenates and cell lysates.

AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative multiplexing assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 545 nm.

AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.

规格

检测目标 α-tubulin
检测目标类 Protein
自动化兼容 Yes
检测方法 Alpha
产品品牌名称 AlphaPlex
运输条件 干冰
Target Species Human
产品尺寸 100 Assay Points