Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.
AequoScreen® Double Transfected Cell Lines: Adrenergic, alpha1A subtype. Human Recombinant, in CHO-K1 host cell. Two vials of cryopreserved cells are shipped per order. A detailed technical dossier includes sequence, culture conditions and pharmacological properties of the recombinant receptor. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some products are not available in some countries. Please inquire at your local sales office for more information.
The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
免责声明: This product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
|检测验证||Binding, Calcium Luminescence|
|治疗领域||Central Nervous System, Metabolic|
|产品尺寸||5 million cells|
|应用文献||AequoScreen aequorin-encoding Plasmids for performing aequorin luminescent assay starting from non-aequorin cell lines||PDF 607 KB|
|单页||Aequorin FLIPR||PDF 1 MB|
|海报||Running an Aequorin Cell-Based Luminescent Assay on the FLIPR TETRA||PDF 270 KB|