PerkinElmer
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AequoZen frozen cells Starter Kit

AequoZen cells expressing the histamine H1 or the muscarinic M5 receptor for flash-luminescence assay.

部件号 ES-001-AF
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This kit contains living cells. Some AequoZen™ FroZen Cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.

详情 信息

AequoZen cells expressing the histamine H1 or the muscarinic M5 receptor for flash-luminescence assay. Recombinant, in CHO-K1 host cell. The kit contains two vials of cryopreserved H1 cells (strong calcium response) and four vials of cryopreserved M5 cells (weaker calcium response). A detailed user manual includes protocols and pharmacological properties of each recombinant receptor enabling you to become familiar with the AequoScreen technology. Please inquire at your local sales office for more information. You are authorized to utilize these frozen cell preparations one time only. Any attempt to transfer, re-use, or propagate these cells is expressly unauthorized and a violation of the product terms and conditions of sale.

规格

检测目标类型 Cell line
检测验证 Calcium Luminescence
宿主细胞 CHO-K1
产品品牌名称 AequoZen
第二信使释放 Calcium flux
运输条件 干冰
治疗领域 Central Nervous System, Pain, Cardiovascular, Inflammation, Gastrointestinal
产品尺寸 1 kit
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应用文献

AequoScreen aequorin-encoding Plasmids for performing aequorin luminescent assay starting from non-aequorin cell lines

Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.

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AequoZen Frozen Cells Starter Kit

G protein coupled receptors (GPCRs) have been considered as a highly “druggable” target for many years, with over 40% of marketed drugs acting to modulate their function. For many years, radiometric techniques have dominated GPCR screening. However in the last decade the development of functional assays, where the effect of molecules is evaluated in terms of GPCR activation, has accelerated.In particular, measurement of calcium signaling and the development of molecular strategies which couple the majority of GPCRs to calcium signaling has allowed the use of high-throughput functional screening in GPCR research.

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