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Anti-bovine IgG2 AlphaLISA Acceptor Beads, 250 µg

AlphaLISA Acceptor beads conjugated to anti-bovine IgG2 antibody. These beads can be used to capture bovine IgG2 antibodies.




部件号 产品尺寸
AL167C 250 µg
AL167M 5 mg
AL167R 25 mg



AlphaLISA Acceptor beads conjugated to anti-bovine IgG2 antibody. These beads can be used to capture bovine IgG2 antibodies. These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash immunoassays for:

  • Antibody-antigen binding studies

  • Analyte detection assays

  • Biomarker detection assays

  • Antibody detection assays

  • Other immunoassays

In a typical AlphaLISA assay, 1 mg of AlphaLISA Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL final reaction volume. Bead concentration can be adjusted for optimal performance.

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

免责声明:  For research use only. Not for use in diagnostic procedures.


抗体偶联物 Anti-bovine IgG2
自动化兼容 Yes
珠型或核心珠型 AlphaLISA Acceptor
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
产品尺寸 250 µg


所有 (7)
资源类型 文件名 文件格式
指南 Alpha Protein-Protein Interaction Quick Start Guide PDF  380 KB
白皮书 Alpha Technologies for Antibody Detection and Characterization PDF  534 KB
产品手册 Alpha product listing PDF  128 KB
指南 AlphaLISA immunoassay development quick guide PDF  1 MB
指南 ELISA to AlphaLISA Immunoassay Conversion Guide PDF  1 MB
指南 User's Guide To Alpha Assays Protein:Protein Interactions PDF  3 MB
活动 Society of Laboratory Automation & Screening (SLAS)