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AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.




部件号 产品尺寸
ALSU-PAKT-B-HV 100 assay points
ALSU-PAKT-B500 500 assay points
ALSU-PAKT-B10K 10,000 assay points
ALSU-PAKT-B50K 50,000 assay points




  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA® SureFire® Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening


分析途径 PI3K/Akt/mTOR
检测目标 Akt1/2/3
检测目标类 Phosphoprotein
自动化兼容 Yes
检测方法 Alpha
产品品牌名称 AlphaLISA SureFire Ultra
包装量 500.0 Units
运输条件 蓝冰
Target Species Human
产品尺寸 500 assay points



所有 (6)
资源类型 文件名 文件格式
单页 Alpha SureFire Technology PDF  720 KB
产品手册 Alpha product listing PDF  128 KB
应用文献 Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra PDF  2 MB
操作手册 Protocol for AlphaLISA SureFire Ultra Akt 1/2/3 (p-Ser473) Assay Kits PDF  769 KB
海报 Rapid homogeneous detection systems for cellular signaling pathways PDF  805 KB
活动 Society of Laboratory Automation & Screening (SLAS)