PerkinElmer
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AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit - High Volume

The AlphaLISA® SureFire® Ultra p-STAT3 (Tyr705) assay is a sandwich style immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.

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部件号
产品尺寸
ALSU-PST3-A-HV
100 assay points
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ALSU-PST3-A500
500 assay points
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ALSU-PST3-A10K
10,000 assay points
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ALSU-PST3-A50K
50,000 assay points
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详情 信息

Formats:

  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.

In the AlphaLISA SureFire Ultra p-STAT3 (Tyr705) assay, donor beads are coated with streptavidin to capture one of the antibodies used in the assay, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, leading to signal generation. The intensity of the light emission is directly proportional to the amount of phosphoprotein present in the sample, making the AlphaLISA SureFire Ultra an excellent immunoassay for quantitative detection of phospho-STAT3 in cellular lysates.

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) kits are compatible with cell and tissue lysates, antibody modulators, and biotherapeutic antibodies. These kits can be used for cellular kinase assays, receptor activation studies, and screening.

规格

分析途径 JAK/STAT
检测目标 STAT3
检测目标类 Phosphoprotein
自动化兼容 Yes
检测方法 Alpha
产品品牌名称 AlphaLISA SureFire Ultra
包装量 100.0 Units
运输条件 蓝冰
Target Species Human
产品尺寸 100 assay points