The AlphaLISA® SureFire® Ultra™ Total SYK assay is a sandwich immunoassay for quantitative detection of SYK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
|ALSU-TSYK-A-HV||100 Assay Points|
|ALSU-TSYK-A500||500 Assay Points|
|ALSU-TSYK-A10K||10,000 Assay Points|
|ALSU-TSYK-A50K||50,000 Assay Points|
In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
免责声明: For research use only. Not for use in diagnostic procedures.
|产品品牌名称||AlphaLISA SureFire Ultra|
|产品尺寸||500 Assay Points|
|单页||Alpha SureFire Technology||PDF 720 KB|
|产品手册||Alpha product listing||PDF 128 KB|
|应用文献||Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra||PDF 2 MB|
|操作手册||Protocol for AlphaLISA SureFire Ultra Total SYK Kits||PDF 743 KB|
|海报||Rapid homogeneous detection systems for cellular signaling pathways||PDF 805 KB|