AlphaLISA® SureFire® Ultra Total p38 MAPK assays are sandwich immunoassays for quantitative detection of p38 MAPK (phosphorylated and unphosphorylated) in cellular lysates using Alpha no-wash technology. This assay can be used for normalization of phosphorylation and other studies.
|ALSU-TP38-A-HV||100 assay points|
|ALSU-TP38-A500||500 assay points|
|ALSU-TP38-A10K||10,000 assay points|
|ALSU-TP38-A50K||50,000 assay points|
The AlphaLISA® SureFire® Ultra™ Total p38 assay is a sandwich immunoassay for quantitative detection of p38 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA® SureFire® Ultra™ kits are compatible with:
Alpha SureFire® kits can be used for:
|产品品牌名称||AlphaLISA SureFire Ultra|
|产品尺寸||10,000 assay points|
|单页||Alpha SureFire Technology||PDF 720 KB|
|产品手册||Alpha product listing||PDF 124 KB|
|应用文献||Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra||PDF 2 MB|
|操作手册||Protocol for AlphaLISA SureFire Ultra Total p38 MAPK Assay Kits||PDF 784 KB|
|海报||Rapid homogeneous detection systems for cellular signaling pathways||PDF 805 KB|
|活动||Society of Laboratory Automation & Screening (SLAS)|