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AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

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部件号
产品尺寸
TGRAF2S500
500 assay points
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TGRAF2S10K
10,000 assay points
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TGRAF2S50K
50,000 assay points
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详情 信息

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Formats:

  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

规格

分析途径 MAPK
检测目标 ATF2
检测目标类 Phosphoprotein
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
分子修饰 Phosphorylation
产品品牌名称 AlphaScreen SureFire
包装量 10000.0 Units
第二信使释放 Protein phosphorylation
运输条件 蓝冰
Target Species Human
产品尺寸 10,000 assay points
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应用文献

A Practical Approach to Cell Signaling Pathway Analysis

Cellular kinase signal transduction pathways are involved in the regulation of many important cellular processes suchas cell survival, differentiation and apoptosis. Kinase signaling networks are typically characterized by multiple kinases arranged in cascades containing nodes with feedback loops, and crosstalk between pathways.

PDF 1 MB
Quick Guide to AlphaScreen SureFire Assay Optimization

Screening kinase activities in cell-based assays offers advantages over the more traditional biochemical approach of using a purified recombinant enzyme to phosphorylate a substrate, since a cell-based assay gives information on a compound’s activity in a more biological context. However, getting initial results with the AlphaScreen® SureFire® assay is highly dependent on optimal cell culture conditions, and often requires that multiple parametersbe optimized in the first set of experiments.

PDF 307 KB

技术说明

Cell lines validated for use with AlphaScreen® SureFire® kits

Cell lines validated for use with AlphaScreen® SureFire® kits

PDF 55 KB