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AlphaScreen SureFire InsulinR (p-Tyr1150;Tyr1151) Assay Kit - 10,000 Points

The AlphaScreen® SureFire® p-Insulin Receptor (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-Insulin Receptor (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

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部件号
产品尺寸
TGRIRS500
500 assay points
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TGRIRS10K
10,000 assay points
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TGRIRS50K
50,000 assay points
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詳情 信息

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Formats:

  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

规格

分析途径 Receptor signaling
检测目标 INSR
检测目标类 Phosphoprotein
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
分子修饰 Phosphorylation
产品品牌名称 AlphaScreen SureFire
包装量 10000.0 Units
第二信使释放 Protein phosphorylation
运输条件 蓝冰
Target Species Human
产品尺寸 10,000 assay points