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AlphaScreen SureFire p-Smad3 (Ser423/425) research kit, 50,000 points

The AlphaScreen® SureFire® p-SMAD3 (p-Ser423/425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/425) in cellular lysates using Alpha Technology.




部件号 产品尺寸
TGRSM3S500 500 assay points
TGRSM3S10K 10,000 assay points
TGRSM3S50K 50,000 assay points



Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening


  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.


分析途径 TGFß/SMAD
检测目标 SMAD3
检测目标类 Phosphoprotein
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
分子修饰 Phosphorylation
产品品牌名称 AlphaScreen SureFire
第二信使释放 Protein phosphorylation
运输条件 蓝冰
Target Species Human
产品尺寸 50,000 assay points


所有 (7)
资源类型 文件名 文件格式
单页 Alpha SureFire Technology PDF  720 KB
产品手册 Alpha Technology Solutions PDF  4 MB
产品手册 Alpha product listing PDF  124 KB
操作手册 AlphaScreen® SureFire® phospho-Smad-3 (Ser423/425) Kit PDF  96 KB
操作手册 Protocol for AlphaScreen® SureFire® SMAD3 (p-Ser423/425) Assay Kits PDF  368 KB
应用文献 Quick Guide to AlphaScreen SureFire Assay Optimization PDF  307 KB
活动 Society of Laboratory Automation & Screening (SLAS)