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AlphaScreen SureFire Total Akt1 research kit, 500 points

The AlphaScreen® SureFire® Akt 1 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation.




部件号 产品尺寸
TGRTA1S500 500 assay points
TGRTA1S10K 10,000 assay points
TGRTA1S50K 50,000 assay points



Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening


  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.


分析途径 PI3K/Akt/mTOR
检测目标 Akt1
检测目标类 Protein
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaScreen SureFire
运输条件 蓝冰
Target Species Human
产品尺寸 500 assay points


所有 (7)
资源类型 文件名 文件格式
单页 Alpha SureFire Technology PDF  720 KB
产品手册 Alpha Technology Solutions PDF  4 MB
产品手册 Alpha product listing PDF  124 KB
操作手册 AlphaScreen® SureFire® total-Akt 1 Kit PDF  142 KB
操作手册 Protocol for AlphaScreen® SureFire® Akt1 Totak Assay Kits PDF  422 KB
应用文献 Quick Guide to AlphaScreen SureFire Assay Optimization PDF  307 KB
活动 Society of Laboratory Automation & Screening (SLAS)