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AlphaScreen SureFire GAPDH Assay Kit - 10,000 Points

The AlphaScreen® SureFire® total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a cellular normalization assay.

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部件号 产品尺寸
TGRGDS500 500 assay points
TGRGDS10K 10,000 assay points
TGRGDS50K 50,000 assay points
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概览

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Formats:

  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

规格

检测目标 GAPDH
检测目标类 Phosphoprotein
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
分子修饰 Phosphorylation
产品品牌名称 AlphaScreen SureFire
包装量 10000.0 Units
第二信使释放 Protein phosphorylation
运输条件 蓝冰
Target Species Human
产品尺寸 10,000 assay points

资源,活动及更多信息

资源类型 文件名 文件格式
单页 Alpha SureFire Technology PDF  720 KB
产品手册 Alpha product listing PDF  128 KB
操作手册 Protocol for AlphaScreen® SureFire® GAPDH Assay Kits PDF  348 KB
活动 Society of Laboratory Automation & Screening (SLAS)