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AlphaScreen SureFire Total p70 S6K Assay Kit - 500 Points

The AlphaScreen® SureFire® total p70 S6K assay is a sandwich immunoassay for quantitative detection of p70 S6K (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

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部件号
产品尺寸
TGRT70S500
500 assay points
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TGRT70S10K
10,000 assay points
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TGRT70S50K
50,000 assay points
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詳情 信息

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Formats:

  • The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
  • The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.
  • The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.
  • The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.

规格

分析途径 PI3K/Akt/mTOR
检测目标 p70 S6K
检测目标类 Protein
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaScreen SureFire
运输条件 蓝冰
Target Species Human
产品尺寸 500 assay points