PerkinElmer
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ANGPT1 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human ANGPT1 enables the quantitative determination of human Angiopoietin 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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部件号 产品尺寸
AL3014HV 100 Assay Points
AL3014C 500 Assay Points
AL3014F 5,000 Assay Points
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概览

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Angiopoietin belongs to a family of vascular growth factors. There are several angiopoietins: ANGPT1, ANGPT2, ANGPT4 now identified, and a number of closely related proteins: ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4, ANGPTL5, ANGPTL6, and ANGPTL7. Angiopoietin 1(Ang-1; also ANGPT1) is a 70?kDa glycoprotein which contains 498 amino acids, including a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. It plays a critical role in vessel maturation, adhesion, migration, and survival. This kit has been designed for the detection of Human Angiopoietin 1 in serum and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

免责声明:  For research use only. Not for use in diagnostic procedures.

规格

检测目标 ANGPT1
检测目标类 Protein
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Human
治疗领域 Angiogenesis
产品尺寸 500 Assay Points

资源,活动及更多信息

资源类型 文件名 文件格式
产品手册 Alpha product listing PDF  128 KB
操作手册 AlphaLISA Human ANGPT1 Detection Kit AL3014 PDF  511 KB
活动 Society of Laboratory Automation & Screening (SLAS)