PerkinElmer
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AlphaLISA Anti-mono-methyl-Histone H4 Lysine 20 Acceptor Beads, 5 mg

AlphaLISA® Acceptor beads conjugated to an antibody against Histone H4 that is mono-methylated at lysine 20 (H4K20me1). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL145C
250 µg
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AL145M
5 mg
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AL145R
25 mg
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詳情 信息

AlphaLISA® Acceptor beads designed to detect human Histone H4 that is mono-methylated at lysine 20 (H4K20me1) in a homogeneous AlphaLISA assay. Broad species cross-reactivity is expected based on sequence similarity. Source of antibody: monoclonal.

The anti-mono-methyl Histone H4 Lysine 20 beads were used for the development and optimization of a SET8 methyltransferase assay using a biotinylated Histone H4 as substrate. A technical note describing the assay is available in our product literature.

Features:

  • No-wash epigenetic assay
  • Fully-validated mark specificity
  • Substrate flexibility (peptide, protein, histone, nucleosome substrates)
  • Easy-to-automate
  • Fast assay optimization

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

规格

抗体偶联物 Anti-H4K20me1
自动化兼容 Yes
珠型或核心珠型 AlphaLISA Acceptor
检测方法 Alpha
实验类型 In vitro
分子修饰 Methylation
产品品牌名称 AlphaLISA
运输条件 蓝冰
产品尺寸 5 mg