Biotinylated antibody against human histone H3 that is dimethylated on Lysine 79. This antibody is intended for use in AlphaLISA® no-wash epigenetic assays using DOT1L methyltransferase with oligonucleosome substrates.
For research use only. Not for use in diagnostic procedures.
AlphaLISA® antibody designed to detect Human Histone H3 that is dimethylated on Lysine 79 in a homogeneous AlphaLISA assay. Broad species cross-reactivity is expected based on sequence similarity. Source of antibody: monoclonal. The biotin-anti-dimethyl-Histone H3 Lysine 79 (H3K79me2) AlphaLISA antibody was used for the development and optimization of a DOT1L methyltransferase assay using oligonucleosomes as substrate. A technical note describing the assay is available in our product literature.
This AlphaLISA immunodetection assay measures the methylation at lysine 79 of nucleosomal Histone H3 using anti-histone H3 (C-ter) Acceptor Beads and a biotinylated anti-dimethyl-histone H3 lysine 79 (H3K79me2) antibody.