PerkinElmer
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IgG (bovine) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgG (total) in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

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部件号 产品尺寸
AL530HV 100 assay points
AL530C 500 assay points
AL530F 5,000 assay points
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概览

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In bovine, the IgG class is further divided into two subclasses: IgG1 and IgG2. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. IgG is involved in response to a foreign antigen. The presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, it can bind to many pathogens and also plays an important role in antibody-dependent cell-mediated cytotoxicity. Typically, bovine serum and plasma samples contain about 17 to 27 mg/ml of IgG. The present kit permits detection of Total Bovine IgG (i.e. analyte) in bovine serum and plasma.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

免责声明:  For research use only. Not for use in diagnostic procedures.

规格

检测目标 IgG
检测目标类 Antibody
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
Target Species Bovine
治疗领域 Biologics/Bioprocess
产品尺寸 100 assay points

资源,活动及更多信息

资源类型 文件名 文件格式
产品手册 Alpha product listing PDF  124 KB
应用文献 Antigen-Stimulated PBMC Cytokine Release Measured by AlphaLISA Bovine Cytokine Kits PDF  3 MB
数据单表 Manual AlphaLISA Bovine Total IgG AL530 PDF  266 KB
活动 Society of Laboratory Automation & Screening (SLAS)