The AlphaLISA immunoassay kit for detection and quantitation of bovine TNFα in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
|AL534HV||100 assay points|
|AL534C||500 assay points|
|AL534F||5,000 assay points|
TNFα is a polypeptide hormone mainly produced by activated macrophages and T cells. It is one of the most powerful mediators of inflammation, either playing a beneficial or deleterious role in disease progression. In particular, TNFα plays a clinically-relevant role as a mediator of immune responses associated to mastitis, endotoxic shock and bovine tuberculosis caused by Mycoplasma bovis. The present kit permits detection of bovine TNFα (i.e. analyte) in bovine serum, plasma, and cell culture supernatants.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
免责声明: For research use only. Not for use in diagnostic procedures.
|产品尺寸||100 assay points|
|产品手册||Alpha product listing||PDF 124 KB|
|应用文献||Antigen-Stimulated PBMC Cytokine Release Measured by AlphaLISA Bovine Cytokine Kits||PDF 3 MB|
|数据单表||Manual AlphaLISA Bovine TNFa AL534||PDF 225 KB|
|活动||Society of Laboratory Automation & Screening (SLAS)|