PerkinElmer
check quantity

IL-1α (bovine) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1α in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.

项目已成功添加到购物车

For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL537HV
100 assay points
更多
AL537C
500 assay points
更多
AL537F
5,000 assay points
更多
立即购买

请输入有效的数量

请登录后再添加至收藏夹

购物车为空

詳情 信息

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

IL-1a is a cytokine produced mainly by immune cells. In particular, IL-1a plays a clinically-relevant role as a mediator of inflammatory responses associated with various diseases such as mastitis, endotoxic shock and bovine tuberculosis. The present kit permits detection of bovine IL-1a (i.e. analyte) in bovine serum, plasma, and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

规格

检测目标 IL1α
检测目标类 Cytokine
自动化兼容 Yes
检测方法 Alpha
产品品牌名称 AlphaLISA
包装量 100.0 Units
运输条件 蓝冰
Target Species Bovine
治疗领域 Inflammation
产品尺寸 100 assay points