The AlphaLISA® immunoassay kit for human BTLA enables the quantitative determination of human B- and T-lymphocyte attenuator (BTLA, CD272) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
|AL3062HV||100 Assay Points|
|AL3062C||500 Assay Points|
|AL3062F||5,000 Assay Points|
AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
B- and T-lymphocyte attenuator (BTLA), also known as CD272, is a 35 kDa transmembrane protein that is expressed on Th1 cells, B cells and NK cells. BTLA's main function is to regulate T cell activation by acting as an inhibitor when interacting with HVEM, B7-H4 and TNF receptors. Deletion of BTLA results in the overproduction of T cells, especially CD8+ cancer specific memory T cells. Higher expression of BTLA is correlated to patients with lung cancer and sepsis. This kit is designed to detect and quantify the levels of BTLA in cell culture supernatant and serum.
免责声明: For research use only. Not for use in diagnostic procedures.
|产品尺寸||5,000 Assay Points|
|产品手册||Alpha product listing||PDF 124 KB|
|技术说明||AlphaLISA Assays for Immune Checkpoint Detection and Quantitation||PDF 532 KB|
|应用文献||Development of an AlphaLISA Assay for Screening Inhibitors of the TIM-3/Gal-9 Interaction||PDF 3 MB|
|操作手册||Manual: AlphaLISA BTLA Detection Kit AL3062||PDF 506 KB|
|应用文献||Protocol Optimization for Detection and Quantification of Membrane-Bound Proteins Using AlphaLISA Te||PDF 2 MB|
|应用文献||Quantifying Changes in CD28 and CTLA-4 Levels in Peripheral Blood Mononuclear Cells with AlphaLISA||PDF 1 MB|
|应用文献||Utilizing AlphaLISA Technology to Screen for Inhibitors of the CTLA-4 Immune Checkpoint||PDF 1 MB|