PerkinElmer
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C-peptide (human) AlphaLISA Kit, 500 Assay Points

AlphaLISA® no-wash immunoassay kit for detection of human C-peptide (human connecting peptide) in serum, buffered solution or cell culture medium.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL299C
500 assay points
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AL299F
5,000 assay points
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詳情 信息

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Mature Connecting Peptide (C-Peptide) is a 31 amino acid peptide that is a product from the proteolytic processing of proinsulin. Through its G-protein coupled membrane receptor, C-Peptide activates Ca2+-dependent intracellular signaling pathways, resulting in subsequent activation of both Na+/K+ ATPase and endothelial nitric oxide synthase. C-Peptide and insulin are co-secreted in equimolar amounts in the bloodstream by pancreatic beta cells of the islets of Langerhans. However, C-Peptide has a longer half-life than insulin in serum, making it a good indicator of endogenous insulin production. A lack of endogenous C-Peptide contributes to development of long-term microvascular complications in the nerves, the kidneys, and the retina in Type 1 diabetes patients. C-Peptide has been considered as a possible therapy to prevent long-term complications of diabetes.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

规格

检测目标 C-peptide
检测目标类 Peptide
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Human
治疗领域 Biologics/Bioprocess
产品尺寸 500 assay points