PerkinElmer
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Corticosterone (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human corticosterone enables the quantitative determination of corticosterone in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL3020HV
100 Assay Points
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AL3020C
500 Assay Points
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AL3020F
5,000 Assay Points
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詳情 信息

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Corticosterone (CORT) is a member of corticosteroids and is also known as 17-deoxycortisol or 11β 21-dihydroprogesterone. CORT is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone and is the precursor of pregnenolone, cortisol, and aldosterone. CORT production has been shown to increase with stress and the elevated CORT levels have been associated with impairment of long term memory retrieval. In some species, CORT is a main glucocorticoid, involved in regulation of energy, immune reactions, and stress responses. Furthermore, CORT serves as a major homeostatic modulator of sodium and potassium levels in vivo.

AlphaLISA technology allows the detection of molecules of interest in buffer and in serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. Biotinylated corticosterone-BSA complex and free corticosterone (in standards or in samples) compete for binding to anti-corticosterone antibody that is conjugated to AlphaLISA Acceptor beads. In the presence of Streptavidin-coated Alpha Donor beads, biotinylated Corticosterone-BSA complex binds to the Streptavidin-coated Alpha Donor beads. When corticosterone-BSA binds to anti-corticosterone AlphaLISA acceptor beads, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

规格

检测目标 Corticosterone
检测目标类 Hormone
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Human
治疗领域 Metabolic
产品尺寸 100 Assay Points