Cry1Ab/1Ac AlphaLISA Detection Kit, 500 Assay Points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Cry1Ab and Cry1Ac are insecticidal proteins produced by the naturally occurring soil bacterium Bacillus thuringiensis subsp. kurstaki (Btk). The genes encoding Cry1Ab and Cry1Ac have been widely introduced to genomes of many crops (e.g. corn, cotton, and soybean) to create genetically modified organisms (GMO). During plant growth, genetically modified crops produce the introduced Cry proteins that confer protection against certain insect pests. The present kit allows the detection of Cry1Ab and Cry1Ac (i.e. analyte) in GMO seeds, leaves, and plant extracts.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.