GAD67 (Human) AlphaLISA Detection Kit, 500 Assay Points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Glutamic acid decarboxylase 67 (GAD67) is an enzyme that catalyzes more than 90% of γ-amino butyric acid by glutamate decarboxylation. GAD67 is expressed in the brain and must be active at all times for normal cellular functioning. Dysregulation of GAD67 in the hippocampal stratum oriens layer correlates to the pathophysiology of schizophrenia, bipolar disorders, and Parkinson’s. This enzyme, as well as GAD65, is a target of autoantibodies in humans who develop type 1 diabetes later in their life. This AlphaLISA detection kit allows for the detection of GAD67 levels in serum, plasma, and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.