PerkinElmer
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FCGR3A/CD16A (176Phe/F158) (human) AlphaLISA Detection Kit, 100 assay points

This kit is designed for the detection of binding activity between human FCGR3A (176Phe) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.

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部件号 产品尺寸
AL347HV 100 assay points
AL347C 500 assay points
AL347F 5,000 assay points
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概览

This kit is designed for the detection of binding activity between human FCGR3A (176Phe) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.


Features:



  • No-wash steps, no separation steps

  • Ease-of-use: few addition steps, fast assay development

  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity

  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more

  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders


These kits use IgG Fc AlphaLISA® Acceptor beads and Streptavidin-coated Donor beads to capture biotinylated human FCGR3A in a competition assay. Donor beads and Acceptor beads come into proximity through IgG Fc fragment binding to FCGR3A. Excitation of the Donor beads provokes the release of singlet oxygen that triggers a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.


The Fc-Gamma Receptors (FCGRs) are members of immunoglobulin superfamily and play a critical role in the function of therapeutic antibodies. FCGRs are divided into three classes and FCGR3 (CD16) is expressed as two distinct forms (FCGR3A and FCGR3B) encoded by two different highly homologous genes in a cell type specific manner. FCGR3A is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), mast cell degranulation and clearance of immune complexes. In humans, a single nucleotide polymorphism creates two isoforms: high binding (176Val/V158) and low binding (176Phe/F158) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies. FCGR3A has been considered as an important therapeutic target. This AlphaLISA assay can be used to determine the binding activity of human IgG Fc fragment to human FCGR3A and also can be used to study how other antibodies bind to FCGR3A by competition assay.

免责声明:  For research use only. Not for use in diagnostic procedures.

规格

检测目标 FCGR3A
检测目标类 Fc-receptor
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Human
治疗领域 Biologics/Bioprocess
产品尺寸 100 assay points

资源,活动及更多信息

资源类型 文件名 文件格式
产品手册 Alpha product listing PDF  128 KB
数据单表 AlphaLISA FCGR3A/CD16a (176Phe/F158) Binding Kit PDF  576 KB
应用文献 Fc-Receptor Binding ADCC Assays Utilizing AlphaLISA: Characterization of hIgGs and FcγRIIIa PDF  1 MB
活动 Society of Laboratory Automation & Screening (SLAS)