Pre-formulated buffer that can be used as a high blocking buffer for Alpha analyte detection assays. This buffer is included in select AlphaLISA immunoassay kits, but can also be purchased to develop your own Alpha assay.
For research use only. Not for use in diagnostic procedures.
Several pre-formulated buffers are available for Alpha assays. These have have been developed to meet the varying needs of different Alpha assays, and one of them may be a good choice for an assay that you are developing. This buffer is an alternative to AlphaLISA Immunoassay Buffer, and should be used in situations where high background from sample components could interfere with the immunoassay.
10X Formulation: 250 mM HEPES, pH 7.4, 1% Casein, 10 mg/mL Dextran-500, 5% Triton X-100, 5% Blocking reagent, 5% BSA and 0.5% Proclin-300.