Pre-formulated buffer that can be used as a high blocking buffer for Alpha analyte detection assays. This buffer is included in select AlphaLISA immunoassay kits, but can also be purchased to develop your own Alpha assay.
For research use only. Not for use in diagnostic procedures.
Several pre-formulated buffers are available for Alpha assays. These have have been developed to meet the varying needs of different Alpha assays, and one of them may be a good choice for an assay that you are developing. This buffer is an alternative to AlphaLISA Immunoassay Buffer, and should be used in situations where high background from sample components could interfere with the immunoassay.
10X Formulation: 250 mM HEPES, pH 7.4, 1% Casein, 10 mg/mL Dextran-500, 5% Triton X-100, 5% Blocking reagent, 5% BSA and 0.5% Proclin-300.
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Integral plasma membrane (EGFR) and secreted solubleproteins (TNFa, IL1ß, IL6, IL8) were successfully determined, on adherent or suspension cells, using the standard AlphaLISA Immunoassay buffer.
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.
Enzyme-linked Immunosorbent Assay is the most widely Kits adopted method for detection and quantification of cytokines and other biomarkers. This traditional technology offers good,selectivity, sensitivity and assay versatility; however, it has certain disadvantages such as limited dynamic range and low throughput due to the numerous wash steps.