The AlphaLISA® immunoassay kit for human HLA-E enables the quantitative determination of human MHC class I antigen E (HLA-E) in buffered solution, cell culture supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
|AL3057HV||100 Assay Points|
|AL3057C||500 Assay Points|
|AL3057F||5,000 Assay Points|
AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
HLA-E, also known as MHC class I antigen E, official full name as major histocompatibility complex, class I, E, is a protein that in humans is encoded by the HLA-E gene. HLA-E is a heterodimer consisting of a heavy chain and a light chain (β-2 microglobulin). The heavy chain is approximately 45 kDa and anchored in the membrane. HLA-E has a very specialized role in cell recognition by natural killer cells (NK cells). HLA-E binds to CD94/NKG2C to regulate NK cell cytolysis activities. This kit has been designed for the detection of Human HLA-E in cell lysate and cell culture supernatants.
免责声明: For research use only. Not for use in diagnostic procedures.
|产品尺寸||100 Assay Points|
|产品手册||Alpha product listing||PDF 124 KB|
|技术说明||AlphaLISA Assays for Immune Checkpoint Detection and Quantitation||PDF 532 KB|
|应用文献||Development of an AlphaLISA Assay for Screening Inhibitors of the TIM-3/Gal-9 Interaction||PDF 3 MB|
|操作手册||Manual: AlphaLISA HLA-E Detection Kit AL3057||PDF 538 KB|
|应用文献||Protocol Optimization for Detection and Quantification of Membrane-Bound Proteins Using AlphaLISA Te||PDF 2 MB|
|应用文献||Quantifying Changes in CD28 and CTLA-4 Levels in Peripheral Blood Mononuclear Cells with AlphaLISA||PDF 1 MB|
|应用文献||Utilizing AlphaLISA Technology to Screen for Inhibitors of the CTLA-4 Immune Checkpoint||PDF 1 MB|