PerkinElmer
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PIP Collagen (human) AlphaLISA Detection Kit, 500 assay points

The AlphaLISA® Human PIP Detection Kit is designed for detection and quantitation of Human Procollagen Type I C-peptide (PIP) in human serum, plasma, cell culture supernatants, cell lysate, and tissue homogenates using a homogeneous (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL353HV
100 assay points
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AL353C
500 assay points
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AL353F
5,000 assay points
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詳情 信息

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Procollagen Type I C-peptide (PIP), also known as Procollagen I Carboxy Terminal Propeptide (PICP), is an enzymatically cleaved product of pro-collagen type I. Cleavage of PIP is required for the initiation of fibril formation. PIP has been used as an indicator of the synthesis of type I collagen. PIP is a trimeric, globular protein consisting of three polypeptide chains, two proα1 (I) and one proα2 (I) chains with molecular mass of PIP of 100 KD. The levels of PIP in biological samples is directly associated with numerous pathological states including bone, liver, heart diseases. The present kit is designed to detect hPIP in human serum, plasma, cell culture supernatants, cell lysate, and tissue homogenates.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

规格

检测目标 PIP Collagen
检测目标类 Protein
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Human
治疗领域 Cancer
产品尺寸 500 assay points