PerkinElmer
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IL-2 (human) AlphaLISA Biotin-Free Detection Kit, 5,000 Assay Points

AlphaLISA Human IL2 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-2 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL333HV
100 assay points
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AL333C
500 assay points
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AL333F
5,000 assay points
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详情 信息

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features and Benefits:

  • No-wash steps, no separation steps
  • Compatible with high biotin samples, including RPMI and high-biotin tissue extracts
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Results in less than 3 hours

Interleukin 2 (IL-2), formerly known as T-cell growth factor (TCGF), is a 15 kDa immunoregulatory lymphokine known to be produced by lectin- or antigen-activated T cells and capable of inducing the thymic expansion of recently activated antigen-specific T lymphocytes. However, since T-cell immunity could be elicited to various agents in the absence of IL-2 in vivo, it is currently thought that the main non-redundant activity of this cytokine is the induction of the suppressor function of CD4+CD25+ regulatory T cells (Treg) in peripheral lymph nodes to ensure suppression of autoreactive T cells that escape negative selection. Moreover, recent findings suggest that IL-2-mediated regulation of Treg cells is important in the prevention of type 1 diabetes and autoimmune disease.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA biotin-free assay, a DIG-labeled anti-analyte antibody binds to the anti-DIG-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

规格

检测目标 IL2
检测目标类 Cytokine
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Human
治疗领域 Inflammation
产品尺寸 5,000 assay points