PerkinElmer
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Utrophin (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human utrophin enables the quantitative determination of utrophin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL3004HV
100 Assay Points
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AL3004C
500 Assay Points
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AL3004F
5,000 Assay Points
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详情 信息

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Utrophin is a muscle protein found at neuromuscular synapse and myotendinous junctions. It is essential for the maintenance of cellular membrane and cytoskeleton structure. Utrophin expression is dramatically increased in patients with muscle weakness and has been involved in Duchenne's muscular dystrophy. In fact, the Utrophin inhibitors are being tested in clinical trials for the prevention of Duchenne's muscular dystrophy. This kit detects Utrophin in serum, plasma, and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

规格

检测目标 Utrophin
检测目标类 Hormone
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Human
治疗领域 Metabolic
产品尺寸 100 Assay Points