The AlphaLISA® immunoassay kit for bovine IL-12 enables the quantitative determination of bovine IL-12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
|AL553HV||100 Assay Points|
|AL553C||500 Assay Points|
|AL553F||5,000 Assay Points|
Interleukin 12 (IL-12) belongs to IL-12 family consisting IL-12, IL-23, IL-27, IL-35. IL-12 is encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis. In response to antigenic stimulation, IL12 proteins are produced by dendritic cells, macrophages, neutrophils, and B-cells. This cytokine stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. Il12 plays essential role in inflammatory diseases. This kit is designed to detect IL12p40 in bovine serum, plasma, and cell culture supernatants.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
免责声明: For research use only. Not for use in diagnostic procedures.
|产品尺寸||500 Assay Points|
|产品手册||Alpha product listing||PDF 124 KB|
|操作手册||AlphaLISA Bovine IL-12 Detection kit AL553||PDF 406 KB|
|应用文献||Antigen-Stimulated PBMC Cytokine Release Measured by AlphaLISA Bovine Cytokine Kits||PDF 3 MB|
|活动||Society of Laboratory Automation & Screening (SLAS)|