PerkinElmer
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IL-12 (bovine) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for bovine IL-12 enables the quantitative determination of bovine IL-12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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部件号 产品尺寸
AL553HV 100 Assay Points
AL553C 500 Assay Points
AL553F 5,000 Assay Points
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概览

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interleukin 12 (IL-12) belongs to IL-12 family consisting IL-12, IL-23, IL-27, IL-35. IL-12 is encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis. In response to antigenic stimulation, IL12 proteins are produced by dendritic cells, macrophages, neutrophils, and B-cells. This cytokine stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. Il12 plays essential role in inflammatory diseases. This kit is designed to detect IL12p40 in bovine serum, plasma, and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

免责声明:  For research use only. Not for use in diagnostic procedures.

规格

检测目标 IL12
检测目标类 Cytokine
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Bovine
治疗领域 Inflammation
产品尺寸 500 Assay Points

资源,活动及更多信息

资源类型 文件名 文件格式
产品手册 Alpha product listing PDF  124 KB
操作手册 AlphaLISA Bovine IL-12 Detection kit AL553 PDF  406 KB
应用文献 Antigen-Stimulated PBMC Cytokine Release Measured by AlphaLISA Bovine Cytokine Kits PDF  3 MB
活动 Society of Laboratory Automation & Screening (SLAS)