PerkinElmer
check quantity

IP-10 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.

项目已成功添加到购物车

For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL326HV
100 assay points
更多
AL326C
500 assay points
更多
AL326F
5,000 assay points
更多
立即购买

请输入有效的数量

请登录后再添加至收藏夹

购物车为空

詳情 信息

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interferon ?-induced protein 10 (IP10) is also known as IC-X-C motif chemokine 10 (CXCL10) or small-inducible cytokine B10. It is an 8.7 kDa protein encoded by the CXCL10 gene and belongs to the CXC chemokine family. IP10 is secreted by several types of immune cells in response to IFN? stimulation. Increased levels appear to be a pre-treatment marker for interferon/ribavirin therapy in HCV and HIV infected patients. The present kit permits the detection of human IP10 (i.e. analyte) in human serum, plasma, and immune cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

规格

检测目标 IP10
检测目标类 Cytokine
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
包装量 1.0 Units
运输条件 蓝冰
Target Species Human
治疗领域 Inflammation
产品尺寸 5,000 assay points