The AlphaLISA® immunoassay kit for human LAG-3 enables the quantitative determination of human lymphocyte-activation gene 3 (LAG-3) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
|AL3058HV||100 Assay Points|
|AL3058C||500 Assay Points|
|AL3058F||5,000 Assay Points|
AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Lymphocyte-activation gene 3 (LAG-3), also known as CD223, is a cell surface protein that affects T-cell function in many ways. LAG-3 is expressed on natural killer cells, plasmacytoid dendritic cells, B-cells, and activated T-cells. LAG-3 works in similar fashion to CTLA-4 and PD-1 in that it negatively regulates cellular proliferation and Treg suppression. Maturation and activation of dendritic cells is also known to be affected by LAG-3 expression. Soluble forms are in clinical development to activate dendritic cells. Anti-LAG-3 antibodies are undergoing studies for anti-cancer and autoimmune response.
免责声明: For research use only. Not for use in diagnostic procedures.
|产品尺寸||100 Assay Points|
|产品手册||Alpha product listing||PDF 128 KB|
|技术说明||AlphaLISA Assays for Immune Checkpoint Detection and Quantitation||PDF 532 KB|
|操作手册||AlphaLISA human LAG-3 Detection Kit||PDF 450 KB|
|应用文献||Development of an AlphaLISA Assay for Screening Inhibitors of the TIM-3/Gal-9 Interaction||PDF 3 MB|
|应用文献||Protocol Optimization for Detection and Quantification of Membrane-Bound Proteins Using AlphaLISA Te||PDF 2 MB|
|应用文献||Quantifying Changes in CD28 and CTLA-4 Levels in Peripheral Blood Mononuclear Cells with AlphaLISA||PDF 1 MB|
|应用文献||Utilizing AlphaLISA Technology to Screen for Inhibitors of the CTLA-4 Immune Checkpoint||PDF 1 MB|