The AlphaLISA® immunoassay kit for human lactate dehydrogenase subunit A (LDHA) enables the quantitative determination of human LDHA in cell lysates and serum using a homogeneous AlphaLISA assay (no wash steps). Please note this is NOT a cell cytotoxicity assay, as the assay does not measure secreted LDH.
For research use only. Not for use in diagnostic procedures.
Please note this is NOT a cell cytotoxicity assay. Formats:
AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Lactate Dehydrogenase A subunit (LDHA) is one subunit of lactate dehydrogenase, which is a tetramer of A and B subunits. Subunit A is mainly present in muscles, where it plays a key role in energy metabolism by catalyzing the oxidation of pyruvate into lactate to produce energy in absence of oxygen. The enzyme is ubiquitous in cells playing the same metabolic functions. Elevation of the enzyme levels in cells is a known marker of many cancers, while its elevation in cell culture media is indicative of cell death. This detection kit is designed to quantify LDHA in serum and cell lysates.
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