The AlphaLISA® Mouse Interferon-γ (IFNγ) Detection Kit is designed for detection and quantitation of mouse IFNγ in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
|AL501C||500 assay points|
|AL501F||5,000 assay points|
In the mouse, Interferon-gamma (IFN-?) is a homodimeric glycoprotein of 133 amino acids. Interferons (IFNs) were discovered due to their antiviral effects. In humans, there are three families of IFNs: IFN type I (IFN-a, ß, ?, e, and ?), IFN type II (one single representative, IFN-?), and IFN type III (IFN-?1-3). Antigens and mitogens stimulate the production of IFN-? in Natural Killer and activated helper T lymphocytes. IFN-? shows multiple effects. It induces the production of cytokines, upregulates the expression of class I and II MHC antigens and leukocyte adhesion molecules. It also activates macrophages and enhances the secretion of immunoglobulins by B cells. Response to IFN-? is mediated by the heterodimeric IFN-? receptor. Importantly, IFNs have proven to be effective in the treatment of several viral infections and cancers.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
免责声明: For research use only. Not for use in diagnostic procedures.
|产品尺寸||5,000 assay points|
|产品手册||Alpha product listing||PDF 124 KB|
|海报||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|
|数据单表||Manual AlphaLISA Mouse IFN? AL501||PDF 240 KB|
|活动||Society of Laboratory Automation & Screening (SLAS)|