PerkinElmer
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MMP-1 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® Human Matrix Metalloproteinase-1 (MMP1) Detection Kit is designed for detection and quantitation of human MMP1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP1 and pro-MMP1.

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部件号 产品尺寸
AL242C 500 assay points
AL242F 5,000 assay points
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概览

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Matrix Metalloproteinase-1 (MMP1) is a 52 kDa, zinc- and calcium-dependent, endopeptidase expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP1 is secreted as a pro-enzyme (pro-MMP1) and is activated by proteolytic cleavage by serine proteases such as plasmin. MMP1 is involved in the breakdown of all the components of the extracellular matrix during embryonic development, reproduction, and tissue remodeling. In normal conditions, MMP1 participates in tissue remodeling, blood vessel formation, and bone development. The expression level and activity of MMP1 is frequently deregulated in several diseases, cancer and rheumatoid arthritis in particular.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

免责声明:  For research use only. Not for use in diagnostic procedures.

规格

检测目标 MMP1
检测目标类 Protein
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Human
治疗领域 Cancer
产品尺寸 500 assay points