The AlphaLISA® Human Matrix Metalloproteinase-9 (MMP9) Detection Kit is designed for detection and quantitation of human MMP9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP9 and pro-MMP9.
|AL243C||500 assay points|
|AL243F||5,000 assay points|
Matrix Metalloproteinase-9 (MMP9) or gelatinase B is a 64-82 kDa, zinc- and calcium-dependent, endopeptidase secreted by normal alveolar macrophages and granulocytes. MMP9 is secreted as a pro-enzyme (pro-MMP9) and is activated by proteolytic cleavage by serine proteases including other MMPs. MMP9 is believed to act synergistically with MMP1 interstitial collagenase in the degradation of fibrillar collagens. MMP9 might contribute to the pathogenesis of joint destruction. MMP9 could also play a role in bone osteoclastic resorption and cancer metastasis. MMP9 constitutes a useful biomarker of disease status in arthritis and certain cancers.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
免责声明: For research use only. Not for use in diagnostic procedures.
|产品尺寸||5,000 assay points|
|海报||All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures||PDF 288 KB|
|产品手册||Alpha Technology Solutions||PDF 4 MB|
|产品手册||Alpha product listing||PDF 124 KB|
|海报||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|
|海报||Comparison of EMT biomarker expression in 2D monolayer and 3D spheroid cultures in a prostate cancer||PDF 1 MB|
|海报||Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines||PDF 279 KB|
|数据单表||Manual AlphaLISA Human MMP9 AL243||PDF 215 KB|
|活动||Society of Laboratory Automation & Screening (SLAS)|